Effects of Commercial Antioxidants Applied in Ovo on Chorioallantoic Membrane and Putative Plasma Vitellogenin of Philippine Mallard (Anas platyrynchos L.)

Abstract

Two experiments were conducted to evaluate the effects of different antioxidants and its overdose (4x) in ovo. Experiment 1 were grouped as vitamin C, MegaAntioxidant^® and Proflavanol C^®, while experiment 2 were grouped as 100, 200 and 400ppm of MegaAntioxidant^® having positive and negative control for both. The 200 fertile eggs in each experiment were injected with 100ul/egg of treatment solution and placebo (positive control), except negative control. Embryonic weight and body measurements were taken weekly (7-21 d). The anti-angiogenesis using CAM at day 4 was accomplished. Determination of putative vitellogenin (VTG) was done at 28^th day using SDS-PAGE. Both experiments showed comparable embryonic weight, body length, and head diameter indicating no deleterious effects of different antioxidants and MegaAntioxidants^® at high doses. Absence of red blemishes of liver was observed in three different antioxidants. The CAM resulted to lesser blood vessel formation significantly at 200 and 400 ppm MegaAntioxidant^®. Further, the 175 kDa putative VTG, was present in vitamin C, Proflavanol C^®, positive control and negative control, except MegaAntioxidant^®. The results imply that MegaAntioxidant^® having 32 different antioxidants could have protected the liver, spared VTG synthesis and had anti-angiogenic property at 200 and 400ppm doses.