It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
Cryopreservation is used in long term storage and transportation. Human ES / iPS cells could be useful in regenerative medicine. The problem of using cryopreservation for storing and transporting human ES / iPS cells is that the survival rate of these cells is low during the freezing and thawing process. Better cooling rates and cryoprotectants are needed for improvement of the cell survival rate. The growth of the ice crystals causes dehydration, deformation, contraction and increase of the electrolytic concentration. When cooled fast enough the cells freeze in the vitrification state and the ice crystals don’t have time to form. Immersion in liquid nitrogen is necessary to achieve a high cooling rate. To achieve a higher cooling rate than the current state, it was reported in pur previous study that the effect of the surface condition on the cooling rate during cryopreservation was investigated. It was confirmed that the cooling rate is improved by covering the cooling subject with a stainless steel mesh. However, the difference in behavior of the vapor bubble during film boiling with surface condition has not been clarified yet. In order to investigate the boiling state with high cooling rate, visualization of film boiling on the surface of cooling object was performed using a high-speed camera. It was confirmed from the image analysis results that the size and frequency of the vapor bubble were changed with the surface condition.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 National Institute of Technology, Akita College, 1-1 Iijima-Bunkyo-cho, Akita, 011-8511, JAPAN
2 Turku University of Applied Sciences, Joukahaisenkatu 3, Turku, 20520, FINLAND