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Abstract
Enteropathogenic E. coli virulence genes are under the control of various regulators, one of which is PerA, an AraC/XylS-like regulator. PerA directly promotes its own expression and that of the bfp operon encoding the genes involved in the biogenesis of the bundle-forming pilus (BFP); it also activates PerC expression, which in turn stimulates locus of enterocyte effacement (LEE) activation through the LEE-encoded regulator Ler. Monomeric PerA directly binds to the per and bfp regulatory regions; however, it is not known whether interactions between PerA and the RNA polymerase (RNAP) are needed to activate gene transcription as has been observed for other AraC-like regulators. Results showed that PerA interacts with the alpha subunit of the RNAP polymerase and that it is necessary for the genetic and phenotypic expression of bfpA. Furthermore, an in silico analysis shows that PerA might be interacting with specific alpha subunit amino acids residues highlighting the direction of future experiments.
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1 Benemérita Universidad Autónoma de Puebla, Centro de Detección Biomolecular, Puebla, Mexico (GRID:grid.411659.e) (ISNI:0000 0001 2112 2750); Universidad Nacional Autónoma de México, Departamento de Microbiología Molecular, Instituto de Biotecnología, Cuernavaca, Mexico (GRID:grid.9486.3) (ISNI:0000 0001 2159 0001)
2 Universidad Nacional Autónoma de México, Departamento de Microbiología Molecular, Instituto de Biotecnología, Cuernavaca, Mexico (GRID:grid.9486.3) (ISNI:0000 0001 2159 0001); Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Laboratorio de Genética Microbiana, Departamento de Microbiología, Mexico City, Mexico (GRID:grid.418275.d) (ISNI:0000 0001 2165 8782)
3 Benemérita Universidad Autónoma de Puebla, Centro de Detección Biomolecular, Puebla, Mexico (GRID:grid.411659.e) (ISNI:0000 0001 2112 2750); Universidad Nacional Autónoma de México, Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Cuernavaca, Mexico (GRID:grid.9486.3) (ISNI:0000 0001 2159 0001)
4 Benemérita Universidad Autónoma de Puebla, Centro de Detección Biomolecular, Puebla, Mexico (GRID:grid.411659.e) (ISNI:0000 0001 2112 2750)
5 Universidad Nacional Autónoma de México, Departamento de Microbiología Molecular, Instituto de Biotecnología, Cuernavaca, Mexico (GRID:grid.9486.3) (ISNI:0000 0001 2159 0001)
6 Benemérita Universidad Autónoma de Puebla, Centro de Detección Biomolecular, Puebla, Mexico (GRID:grid.411659.e) (ISNI:0000 0001 2112 2750); Benemérita Universidad Autónoma de Puebla, Vicerrectoría de Investigación y Estudios de Posgrado, Puebla, Mexico (GRID:grid.411659.e) (ISNI:0000 0001 2112 2750)
7 Universidad Nacional Autónoma de México, Unidad Académica Yucatán, Instituto de Investigaciones en Matemáticas Aplicadas y en Sistemas, Mérida, Mexico (GRID:grid.9486.3) (ISNI:0000 0001 2159 0001)
8 Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Laboratorio de Genética Microbiana, Departamento de Microbiología, Mexico City, Mexico (GRID:grid.418275.d) (ISNI:0000 0001 2165 8782)