Abstract

The most daunting issue of global climate change is the deleterious impact of extreme temperatures on tea productivity and quality, which has resulted in a quest among researchers and growers. The current study aims to unravel molecular programming underpinning thermotolerance by characterizing heat tolerance and sensitivity response in 20 tea cultivars. The significantly higher negative influence of heat stress was recorded in a sensitive cultivar with reduced water retention (47%), chlorophyll content (33.79%), oxidation potential (32.48%), and increase in membrane damage (76.4%). Transcriptional profiling of most tolerant and sensitive cultivars identified 78 differentially expressed unigenes with chaperon domains, including low and high molecular weight heat shock protein (HSP) and heat shock transcription factors (HSFs) involved in heat shock response (HSR). Further, predicted transcriptional interactome network revealed their key role in thermotolerance via well-co-ordinated transcriptional regulation of aquaporins, starch metabolism, chlorophyll biosynthesis, calcium, and ethylene mediated plant signaling system. The study identified the key role of HSPs (CsHSP90) in regulating HSR in tea, wherein, structure-based molecular docking revealed the inhibitory role of geldanamycin (GDA) on CsHSP90 by blocking ATP binding site at N-terminal domain of predicted structure. Subsequently, GDA mediated leaf disc inhibitor assay further affirmed enhanced HSR with higher expression of CsHSP17.6, CsHSP70, HSP101, and CsHSFA2 genes in tea. Through the current study, efforts were made to extrapolate a deeper understanding of chaperons mediated regulation of HSR attributing thermotolerance in tea.

Details

Title
Underpinning the molecular programming attributing heat stress associated thermotolerance in tea (Camellia sinensis (L.) O. Kuntze)
Author
Seth Romit 1   VIAFID ORCID Logo  ; Maritim Tony Kipkoech 2   VIAFID ORCID Logo  ; Parmar Rajni 1 ; Sharma, Ram Kumar 3   VIAFID ORCID Logo 

 CSIR-Institute of Himalayan Bioresource Technology (CSIR-IHBT), Biotechnology Department, Palampur, India (GRID:grid.417640.0) (ISNI:0000 0004 0500 553X) 
 CSIR-Institute of Himalayan Bioresource Technology (CSIR-IHBT), Biotechnology Department, Palampur, India (GRID:grid.417640.0) (ISNI:0000 0004 0500 553X); Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India (GRID:grid.469887.c) (ISNI:0000 0004 7744 2771); Tea breeding and genetic improvement division, KALRO—Tea Research Institute, Kericho, Kenya (GRID:grid.469887.c) 
 CSIR-Institute of Himalayan Bioresource Technology (CSIR-IHBT), Biotechnology Department, Palampur, India (GRID:grid.417640.0) (ISNI:0000 0004 0500 553X); Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India (GRID:grid.469887.c) (ISNI:0000 0004 7744 2771) 
Publication year
2021
Publication date
2021
Publisher
Oxford University Press
e-ISSN
20527276
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2520052266
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.