It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
Optical diffraction tomography (ODT) enables imaging of unlabeled intracellular components by measuring the three-dimensional (3D) refractive index (RI). We aimed to detect intracellular monosodium urate (MSU) crystals in synovial leukocytes derived from gout patients using ODT. The 3D RI values of the synthetic MSU crystals, measured by ODT, ranged between 1.383 and 1.440. After adding synthetic MSU crystals to a macrophage, RI tomograms were reconstructed using ODT, and the reconstructed RI tomograms discerned intracellular and extracellular MSU crystals. We observed unlabeled synthetic MSU crystal entry into the cytoplasm of a macrophage through time-lapse imaging. Furthermore, using gout patient-derived synovial leukocytes, we successfully obtained RI tomogram images of intracellular MSU crystals. The 3D RI identification of MSU crystals was verified with birefringence through polarization-sensitive ODT measurements. Together, our results provide evidence that this novel ODT can identify birefringent MSU crystals in synovial leukocytes of patients with gout.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 Korea Basic Science Institute (KBSI), Division of Aging Research, Gwangju Center, Gwangju, South Korea (GRID:grid.410885.0) (ISNI:0000 0000 9149 5707); Korea University, Department of Materials Science and Engineering, Seoul, South Korea (GRID:grid.222754.4) (ISNI:0000 0001 0840 2678)
2 Yonsei University College of Medicine, Division of Rheumatology, Department of Internal Medicine, Seoul, South Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454)
3 Yonsei University College of Medicine, Division of Rheumatology, Department of Internal Medicine, Seoul, South Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454); Yonsei University College of Medicine, Synapse Center, Seoul, South Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454)
4 Korea Basic Science Institute (KBSI), Division of Aging Research, Gwangju Center, Gwangju, South Korea (GRID:grid.410885.0) (ISNI:0000 0000 9149 5707)
5 Korea Advanced Institute of Science and Technology (KAIST), Department of Physics, Daejeon, South Korea (GRID:grid.37172.30) (ISNI:0000 0001 2292 0500)
6 Korea University, Department of Materials Science and Engineering, Seoul, South Korea (GRID:grid.222754.4) (ISNI:0000 0001 0840 2678)
7 Korea Advanced Institute of Science and Technology (KAIST), Department of Physics, Daejeon, South Korea (GRID:grid.37172.30) (ISNI:0000 0001 2292 0500); KAIST Institute for Health Science and Technology, KAIST, Daejeon, South Korea (GRID:grid.37172.30) (ISNI:0000 0001 2292 0500); Tomocube Inc., Daejeon, South Korea (GRID:grid.37172.30)
8 Yonsei University College of Medicine, Division of Rheumatology, Department of Internal Medicine, Seoul, South Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454); Yonsei University College of Medicine, Institute for Immunology and Immunological Diseases, Seoul, South Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454)