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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.

Details

Title
Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application
Author
De Benedetti, Stefano 1   VIAFID ORCID Logo  ; Flavio Di Pisa 1 ; Enrico Mario Alessandro Fassi 2   VIAFID ORCID Logo  ; Cretich, Marina 3   VIAFID ORCID Logo  ; Musicò, Angelo 3 ; Frigerio, Roberto 3 ; Mussida, Alessandro 3 ; Bombaci, Mauro 4   VIAFID ORCID Logo  ; Grifantini, Renata 4 ; Colombo, Giorgio 5 ; Bolognesi, Martino 6   VIAFID ORCID Logo  ; Grande, Romualdo 7 ; Zanchetta, Nadia 7 ; Gismondo, Maria Rita 8 ; Mileto, Davide 7 ; Mancon, Alessandro 7 ; Gourlay, Louise Jane 1   VIAFID ORCID Logo 

 Department of Biosciences, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy; [email protected] (S.D.B.); [email protected] (F.D.P.); [email protected] (M.B.) 
 Consiglio Nazionale delle Ricerche, Istituto di Scienze e Tecnologie Chimiche “Giulio Natta” (SCITEC), Via Mario Bianco 9, 20131 Milano, Italy; [email protected] (E.M.A.F.); [email protected] (M.C.); [email protected] (A.M.); [email protected] (R.F.); [email protected] (A.M.); Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, Via L. Mangiagalli 25, 20133 Milano, Italy 
 Consiglio Nazionale delle Ricerche, Istituto di Scienze e Tecnologie Chimiche “Giulio Natta” (SCITEC), Via Mario Bianco 9, 20131 Milano, Italy; [email protected] (E.M.A.F.); [email protected] (M.C.); [email protected] (A.M.); [email protected] (R.F.); [email protected] (A.M.) 
 Istituto Nazionale Genetica Molecolare, Padiglione Romeo ed Enrica Invernizzi, IRCCS Ospedale Maggiore Policlinico, 20122 Milan, Italy; [email protected] (M.B.); [email protected] (R.G.) 
 Dipartimento di Chimica, Università di Pavia, V.le Taramelli 12, 27100 Pavia, Italy; [email protected] 
 Department of Biosciences, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy; [email protected] (S.D.B.); [email protected] (F.D.P.); [email protected] (M.B.); Centro di Ricerca Pediatrica Romeo ed Enrica Invernizzi, Università degli Studi di Milano, 20133 Milano, Italy 
 UOC Microbiologia Clinica, Virologia e Diagnostica delle Bioemergenze ASST FBF Sacco, 20157 Milano, Italy; [email protected] (R.G.); [email protected] (N.Z.); [email protected] (M.R.G.); [email protected] (D.M.); [email protected] (A.M.) 
 UOC Microbiologia Clinica, Virologia e Diagnostica delle Bioemergenze ASST FBF Sacco, 20157 Milano, Italy; [email protected] (R.G.); [email protected] (N.Z.); [email protected] (M.R.G.); [email protected] (D.M.); [email protected] (A.M.); Clinical Microbiology, Virology and Bioemergency Unit, Department of Biomedical and Clinical Sciences, Luigi Sacco Hospital, University of Milan, 20157 Milan, Italy 
First page
322
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
2076393X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2528258211
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.