Full text

Turn on search term navigation

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Human parvovirus (B19V) is the causative agent of erythema infectiosum in children and is linked to a wide range of clinical manifestations. Studies related to B19V prevalence in the Middle East and North Africa (MENA) region and other parts of Asia are very scarce. The objectives of this study were to estimate the seroprevalence (anti-B19V IgM and IgG), the viremia rate (B19V DNA), and the circulating genotypes of B19V among blood donors in Qatar. Methods: Donors’ blood samples (n = 5026) from different nationalities, mainly from the MENA region and South East Asia, were collected from 2014–2016. Samples were tested for the B19V DNA using RT-PCR. Furthermore, 1000 selected samples were tested to determine the seroprevalence of B19V antibodies using enzyme-linked immunosorbent assay (ELISA). Genotyping was performed on 65 DNA positive samples by sequencing of nested PCR fragments (NS1-VP1u region, 927 nt). Results: Only 1.4% (70/5026) of the samples had detectible B19V DNA in their blood. B19V DNA prevalence statistically decreased with age (p = 0.03). Anti-B19V IgG was detected in 60.3% (561/930) of the tested samples, while only 2.1% (20/930) were IgM-positive and 1.2% (11/930) were both IgM- and IgG-positive. B19V genotyping showed a predominance of Genotype 1 (100%). Sequence analysis of the NS1-VP1u region revealed 139 mutation sites, some of which were amino acid substitutions. Conclusion: Our results indicated a relatively high seroprevalence of B19V in Qatar. Most importantly, B19 DNA was detected among Qatari and non-Qatari blood donors. Therefore, blood banks in Qatar might need to consider screening for B19V, especially when transfusion is intended for high-risk populations, including immunocompromised patients.

Details

Title
Prevalence and Phylogenetic Analysis of Parvovirus (B19V) among Blood Donors with Different Nationalities Residing in Qatar
Author
Abdelrahman, Doua 1   VIAFID ORCID Logo  ; Al-Sadeq, Duaa W 2   VIAFID ORCID Logo  ; Smatti, Maria K 3   VIAFID ORCID Logo  ; Taleb, Sara A 4 ; AbuOdeh, Raed O 5 ; Al-Absi, Enas S 6 ; Al-Thani, Asmaa A 7 ; Coyle, Peter V 8 ; Al-Dewik, Nader 9   VIAFID ORCID Logo  ; Al Qahtani, Ahmed A 10 ; Yassine, Hadi M 7   VIAFID ORCID Logo  ; Nasrallah, Gheyath K 7   VIAFID ORCID Logo 

 Department of Human Genetics, Sidra Medicine Doha, Doha, Qatar; [email protected] 
 Biomedical Research Center, Member of QU Health, Qatar University, Doha, Qatar; [email protected] (D.W.A.-S.); [email protected] (M.K.S.); [email protected] (E.S.A.-A.); [email protected] (A.A.A.-T.); [email protected] (H.M.Y.); College of Medicine, Member of QU Health, Qatar University, Doha, Qatar 
 Biomedical Research Center, Member of QU Health, Qatar University, Doha, Qatar; [email protected] (D.W.A.-S.); [email protected] (M.K.S.); [email protected] (E.S.A.-A.); [email protected] (A.A.A.-T.); [email protected] (H.M.Y.); College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar; [email protected] 
 College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar; [email protected] 
 Medical Laboratory Sciences Department, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates; [email protected] 
 Biomedical Research Center, Member of QU Health, Qatar University, Doha, Qatar; [email protected] (D.W.A.-S.); [email protected] (M.K.S.); [email protected] (E.S.A.-A.); [email protected] (A.A.A.-T.); [email protected] (H.M.Y.) 
 Biomedical Research Center, Member of QU Health, Qatar University, Doha, Qatar; [email protected] (D.W.A.-S.); [email protected] (M.K.S.); [email protected] (E.S.A.-A.); [email protected] (A.A.A.-T.); [email protected] (H.M.Y.); Department of Biomedical Science, College of Health Sciences, Member of QU Health, Qatar University, Doha, Qatar 
 Division of Virology, Department of Pathology and Laboratory Medicine, Hamad Medical Corporation, Doha, Qatar; [email protected] 
 Department of Pediatrics, Clinical and Metabolic Genetics, Hamad Medical Corporation, Doha, Qatar; [email protected] 
10  Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh 11564, Saudi Arabia; [email protected]; Department of Microbiology and Immunology, Alfaisal University School of Medicine, Riyadh 11533, Saudi Arabia 
First page
540
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
19994915
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2528259836
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.