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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

Details

Title
Clinical Origin and Species Distribution of Fusarium spp. Isolates Identified by Molecular Sequencing and Mass Spectrometry: A European Multicenter Hospital Prospective Study
Author
Anne-Cécile Normand 1 ; Imbert, Sébastien 2 ; Brun, Sophie 3 ; Al-Hatmi, Abdullah M S 4   VIAFID ORCID Logo  ; Chryssanthou, Erja 5   VIAFID ORCID Logo  ; Cassaing, Sophie 6 ; Schuttler, Christine 7 ; Hasseine, Lilia 8 ; Mahinc, Caroline 9 ; Costa, Damien 10   VIAFID ORCID Logo  ; Bonnal, Christine 11 ; Ranque, Stéphane 12   VIAFID ORCID Logo  ; Sautour, Marc 13 ; Rubio, Elisa 14   VIAFID ORCID Logo  ; Delhaes, Laurence 15 ; Riat, Arnaud 16 ; Sendid, Boualem 17   VIAFID ORCID Logo  ; Kristensen, Lise 18 ; Brandenberger, Marcel 19 ; Guitard, Juliette 20   VIAFID ORCID Logo  ; Packeu, Ann 21 ; Piarroux, Renaud 22   VIAFID ORCID Logo  ; Fekkar, Arnaud 23 

 AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Service de Parasitologie Mycologie, 75013 Paris, France; [email protected] (S.I.); [email protected] (R.P.); [email protected] (A.F.) 
 AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Service de Parasitologie Mycologie, 75013 Paris, France; [email protected] (S.I.); [email protected] (R.P.); [email protected] (A.F.); AP-HP, Hôpital Avicenne, Service de Parasitologie-Mycologie, 93000 Bobigny, France; [email protected]; Centre of Expertise in Mycology Radboud University Medical Centre, Canisius Wilhelmina Hospital, 6525 Nijmegen, The Netherlands; [email protected] 
 AP-HP, Hôpital Avicenne, Service de Parasitologie-Mycologie, 93000 Bobigny, France; [email protected] 
 Centre of Expertise in Mycology Radboud University Medical Centre, Canisius Wilhelmina Hospital, 6525 Nijmegen, The Netherlands; [email protected]; Natural & Medical Sciences Research Center, Department of Microbiology, University of Nizwa, Nizwa 616, Oman 
 Division of Clinical Microbiology, Karolinska Institutet, Department of Laboratory Medicine, 171 77 Stockholm, Sweden; [email protected] 
 CHU Toulouse, Service de Parasitologie-Mycologie, 31000 Toulouse, France; [email protected] 
 Laboratoire Biogroup-LCD, 92300 Levallois, France; [email protected] 
 CHU de Nice, Service de Parasitologie Mycologie, 06200 Nice, France; [email protected] 
 CHU de Saint Etienne, Service de Parasitologie Mycologie, 42000 Saint Etienne, France; [email protected] 
10  Centre Hospitalier Universitaire de Rouen, Service de Parasitologie Mycologie, 76000 Rouen, France; [email protected] 
11  AP-HP, Hôpital Bichat-Claude Bernard, Service de Parasitologie Mycologie, 75018 Paris, France; [email protected] 
12  Aix Marseille University, IRD, AP-HM, SSA, VITROME, IHU Méditerranée Infection, 13005 Marseille, France; [email protected] 
13  CHU de Dijon, Service de Parasitologie Mycologie, 21079 Dijon, France; [email protected] 
14  Department of Microbiology, ISGlobal Barcelona Institute for Global Health, Barcelona, Spain CDB, Hospital Clinic, University of Barcelona, 08036 Barcelona, Spain; [email protected] 
15  CHU de Bordeaux, Groupe Hospitalier Pellegrin, Service de Mycologie, 33404 Bordeaux, France; [email protected] 
16  Laboratory of bacteriology, Division of Laboratory Medicine, University Hospital of Geneva, Rue Gabrielle-Perret-Gentil 4, 1205 Genève, Switzerland; [email protected] 
17  Department Parasitology-Mycology, CHU de Lille, 59000 Lille, France; [email protected] 
18  Department of Clinical Microbiology, Aarhus University Hospital, 8200 Aarhus, Denmark; [email protected] 
19  Labor Synlab Luzern, 6002 Lucerne, Switzerland; [email protected] 
20  Centre de Recherche Saint-Antoine, Inserm, CRSA, AP-HP, Hôpital Saint-Antoine, Service de Parasitologie-Mycologie, Sorbonne Université, 75012 Paris, France; [email protected] 
21  Sciensano, BCCM/IHEM collection, Mycology and Aerobiology Unit, 1000 Brussels, Belgium; [email protected] 
22  AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Service de Parasitologie Mycologie, 75013 Paris, France; [email protected] (S.I.); [email protected] (R.P.); [email protected] (A.F.); Inserm, Institut Pierre Louis d’Epidemiologie et de Santé Publique, Sorbonne Université, 75571 Paris, France 
23  AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Service de Parasitologie Mycologie, 75013 Paris, France; [email protected] (S.I.); [email protected] (R.P.); [email protected] (A.F.); Inserm, CNRS, Centre d’Immunologie et des Maladies Infectieuses, Cimi-Paris, Sorbonne Université, 75005 Paris, France 
First page
246
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
2309608X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2531127042
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.