Competition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell

Abstract

Maintaining functional stability of microbial electrolysis cell (MEC) treating wastewater depends on maintaining functional redundancy of efficient electroactive bacteria (EAB) on the anode biofilm. Therefore, investigating whether efficient EAB competing for the same resources (electron donor and acceptor) co-exist at the anode biofilm is key for the successful application of MEC for wastewater treatment. Here, we compare the electrochemical and kinetic properties of two efficient acetoclastic EAB, Geobacter sulfurreducens (GS) and Desulfuromonas acetexigens (DA), grown as monoculture in MECs fed with acetate. Additionally, we monitor the evolution of DA and GS in co-culture MECs fed with acetate or domestic wastewater using fluorescent in situ hybridization. The apparent Monod kinetic parameters reveal that DA possesses higher j_max (10.7 ± 0.4 A/m²) and lower K_{S, app} (2 ± 0.15 mM) compared to GS biofilms (j_max: 9.6 ± 0.2 A/m² and K_{S, app}: 2.9 ± 0.2 mM). Further, more donor electrons are diverted to the anode for respiration in DA compared to GS. In acetate-fed co-culture MECs, DA (98% abundance) outcompete GS for anode-dependent growth. In contrast, both EAB co-exist (DA: 55 ± 2%; GS: 24 ± 1.1%) in wastewater-fed co-culture MECs despite the advantage of DA over GS based on kinetic parameters alone. The co-existence of efficient acetoclastic EAB with high current density in MECs fed with wastewater is significant in the context of functional redundancy to maintain stable performance. Our findings also provide insight to future studies on bioaugmentation of wastewater-fed MECs with efficient EAB to enhance performance.