Abstract

Sphingosine phosphate lyase 1 (SGPL1) is a highly conserved enzyme that irreversibly degrades sphingosine-1-phosphate (S1P). Sgpl1-knockout mice fail to develop germ cells, resulting in infertility. However, the molecular mechanism remains unclear. The results of the present study showed that SGPL1 was expressed mainly in granulosa cells, Leydig cells, spermatocytes, and round spermatids. Sgpl1 deletion led to S1P accumulation in the gonads. In the ovary, S1P decreased natriuretic peptide receptor 2 (NPR2) activity in granulosa cells and inhibited early follicle growth. In the testis, S1P increased the levels of cyclin-dependent kinase inhibitor 1A (p21) and apoptosis in Leydig cells, thus resulting in spermatogenesis arrest. These results indicate that Sgpl1 deletion increases intracellular S1P levels, resulting in the arrest of female and male germ cell development via different signaling pathways.

Details

Title
Sgpl1 deletion elevates S1P levels, contributing to NPR2 inactivity and p21 expression that block germ cell development
Author
Yuan Feifei 1 ; Wang, Zhijuan 2 ; Sun, Yanli 2 ; Wei, Hongwei 2 ; Cui Yanying 2 ; Wu Zhanying 1 ; Zhang, Chunyu 2 ; Ke-Ping, Xie 2 ; Wang Fengchao 3   VIAFID ORCID Logo  ; Zhang Meijia 2   VIAFID ORCID Logo 

 China Agricultural University, State Key Laboratory for Agrobiotechnology, College of Biological Sciences, Beijing, China (GRID:grid.22935.3f) (ISNI:0000 0004 0530 8290); South China University of Technology, Division of Cell, Developmental and Integrative Biology, School of Medicine, Guangzhou, China (GRID:grid.79703.3a) (ISNI:0000 0004 1764 3838) 
 South China University of Technology, Division of Cell, Developmental and Integrative Biology, School of Medicine, Guangzhou, China (GRID:grid.79703.3a) (ISNI:0000 0004 1764 3838) 
 National Institute of Biological Sciences, Beijing, China (GRID:grid.410717.4) (ISNI:0000 0004 0644 5086) 
Publication year
2021
Publication date
Jun 2021
Publisher
Springer Nature B.V.
e-ISSN
20414889
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2536651769
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.