Synergistic effect of trichloroethylene and Cu(II) on DNA-adduct 8-hydroxy deoxyguanosine (8-OHdG) formation as a biomarker of cancer risk

Abstract

Identification of the synergistic effect of trichloro ethylene and Cu (II) in the formation of DNA adduct 8-hydroxy deoxyguanosine (8-OHdG) as a cancer biomarker were carried out. TCE and CuCl₂ were incubated with deoxyguanosine with variations in temperature (37°C and 60°C) and pH (7.4 and 8.4) for 6 hours. The 8-OHdG levels formed were analyzed using high performance liquid chromatography (HPLC). The formation of 8-OHdG from exposure to TCE seems to require high concentrations, because of its difficulty to oxidize. The addition of Cu (II) was shown to have a synergistic effect with TCE in the formation of DNA adducts which increased up to 65.8%. Temperature did not have a significant effect on DNA adduct formation. Furthermore, it is found that alkaline pH produces more DNA adducts than neutral pH.