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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

(1) Background: Renal development involves frequent expression and loss of transcription factors, resulting in the activation of genes. Wilms’ tumor 1 (WT1), hepatocyte nuclear factor-1-beta (HNF1β), and paired box genes 2 and 8 (Pax2 and Pax8) play an important role in renal development. With this in vivo study, we examined the period and location of expression of these factors in renal development. (2) Methods: Fetal lamb kidneys (50 days from gestation to term) and adult ewe kidneys were evaluated by hematoxylin and eosin staining. Serial sections were subjected to immunohistochemistry for WT1, HNF1β, Pax2, and Pax8. (3) Results: Pax2, Pax8, and HNF1β expression was observed in the ureteric bud and collecting duct epithelial cells. We observed expression of WT1 alone in metanephric mesenchymal cells, glomerular epithelial cells, and interstitial cells in the medullary rays and Pax8 and HNF1β expression in tubular epithelial cells. WT1 was highly expressed in cells more proximal to the medulla in renal vesicles and in C- and S-shaped bodies. Pax2 was expressed in the middle and peripheral regions, and HNF1β in cells in the region in the middle of these. (4) Conclusions: WT1 is involved in nephron development. Pax2, Pax8, and HNF1β are involved in nephron maturation and the formation of peripheral collecting ducts from the Wolffian duct.

Details

Title
The Expression of Transcription Factors in Fetal Lamb Kidney
Author
Nishiya, Yuri 1   VIAFID ORCID Logo  ; Kawaguchi, Kohei 1 ; Kudo, Kosuke 1 ; Kawaguchi, Takuya 1 ; Obayashi, Juma 1   VIAFID ORCID Logo  ; Tanaka, Kunihide 1 ; Ohyama, Kei 1 ; Nagae, Hideki 1 ; Furuta, Shigeyuki 1 ; Seki, Yasuji 1 ; Koike, Junki 2 ; Pringle, Kevin C 3 ; Kitagawa, Hiroaki 1 

 Division of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, Japan; [email protected] (Y.N.); [email protected] (K.K.); [email protected] (K.K.); [email protected] (T.K.); [email protected] (J.O.); [email protected] (K.T.); [email protected] (K.O.); [email protected] (H.N.); [email protected] (S.F.); [email protected] (Y.S.) 
 Department of Pathology, School of Medicine, St. Marianna University, Kawasaki 216-8511, Kanagawa, Japan; [email protected] 
 Department of Obstetrics and Gynecology, School of Medicine & Health Science, University of Otago, North Dunedin, Dunedin 9016, New Zealand; [email protected] 
First page
22
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
22213759
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2544504576
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.