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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

2S albumins are relevant and often major allergens from several tree nuts and seeds, affecting mainly children and young people. The present study aims to assess how the structural features of 2S albumins could affect their immunogenic capacity, which is essential to comprehend the role of these proteins in food allergy. For this purpose, twelve 2S albumins were isolated from their respective extracts by chromatographic methods and identified by MALDI-TOF mass-spectrometry. Their molecular and structural characterization was conducted by electrophoretic, spectroscopic and in silico methods, showing that these are small proteins that comprise a wide range of isoelectric points, displaying a general high structure stability to thermal treatment. Despite low amino acid sequence identity, these proteins share structural features, pointing conformational epitopes to explain cross-reactivity between them. Immunoblotting with allergic patients’ sera revealed those possible correlations between evolutionarily distant 2S albumins from different sources. The availability of a well-characterized panel of 2S albumins from plant-derived sources allowed establishing correlations between their structural features and their allergenic potential, including their role in cross-reactivity processes.

Details

Title
Characterization of Relevant Biomarkers for the Diagnosis of Food Allergies: An Overview of the 2S Albumin Family
Author
Bueno-Díaz, Cristina 1 ; Martín-Pedraza, Laura 2 ; Parrón, Jorge 3 ; Cuesta-Herranz, Javier 4 ; Cabanillas, Beatriz 5   VIAFID ORCID Logo  ; Pastor-Vargas, Carlos 1   VIAFID ORCID Logo  ; Batanero, Eva 1 ; Villalba, Mayte 1   VIAFID ORCID Logo 

 Department of Biochemistry and Molecular Biology, Complutense University of Madrid, 28040 Madrid, Spain; [email protected] (C.B.-D.); [email protected] (J.P.); [email protected] (C.P.-V.); [email protected] (E.B.); RETIC ARADyAL, Health Research Institute Carlos III, 28029 Madrid, Spain; [email protected] (L.M.-P.); [email protected] (J.C.-H.) 
 RETIC ARADyAL, Health Research Institute Carlos III, 28029 Madrid, Spain; [email protected] (L.M.-P.); [email protected] (J.C.-H.); Molecular Allergology Group, Paul-Ehrlich Institut, 63225 Langen, Germany 
 Department of Biochemistry and Molecular Biology, Complutense University of Madrid, 28040 Madrid, Spain; [email protected] (C.B.-D.); [email protected] (J.P.); [email protected] (C.P.-V.); [email protected] (E.B.) 
 RETIC ARADyAL, Health Research Institute Carlos III, 28029 Madrid, Spain; [email protected] (L.M.-P.); [email protected] (J.C.-H.); Health Research Institute Fundación Jiménez Díaz (IIS-FJD, UAM), University Hospital Fundación Jiménez Díaz, 28040 Madrid, Spain 
 Department of Allergy, Research Institute Hospital 12 de Octubre, 28041 Madrid, Spain; [email protected] 
First page
1235
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
23048158
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2544783711
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.