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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Pathogenic fungi and oomycetes give rise to a significant number of animal and plant diseases. While the spread of these pathogenic microorganisms is increasing globally, emerging resistance to antifungal drugs is making associated diseases more difficult to treat. High-throughput screening (HTS) and new developments in lab-on-a-chip (LOC) platforms promise to aid the discovery of urgently required new control strategies and anti-fungal/oomycete drugs. In this review, we summarize existing HTS and emergent LOC approaches in the context of infection strategies and invasive growth exhibited by these microorganisms. To aid this, we introduce key biological aspects and review existing HTS platforms based on both conventional and LOC techniques. We then provide an in-depth discussion of more specialized LOC platforms for force measurements on hyphae and to study electro- and chemotaxis in spores, approaches which have the potential to aid the discovery of alternative drug targets on future HTS platforms. Finally, we conclude with a brief discussion of the technical developments required to improve the uptake of these platforms into the general laboratory environment.

Details

Title
Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes
Author
Sun, Yiling 1   VIAFID ORCID Logo  ; Tayagui, Ayelen 2   VIAFID ORCID Logo  ; Sale, Sarah 3   VIAFID ORCID Logo  ; Sarkar, Debolina 3 ; Nock, Volker 1   VIAFID ORCID Logo  ; Garrill, Ashley 3   VIAFID ORCID Logo 

 Biomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New Zealand; [email protected] (Y.S.); [email protected] (A.T.); [email protected] (S.S.); [email protected] (D.S.); The MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New Zealand 
 Biomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New Zealand; [email protected] (Y.S.); [email protected] (A.T.); [email protected] (S.S.); [email protected] (D.S.); The MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New Zealand; School of Biological Sciences, University of Canterbury, Christchurch 8041, New Zealand 
 Biomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New Zealand; [email protected] (Y.S.); [email protected] (A.T.); [email protected] (S.S.); [email protected] (D.S.); School of Biological Sciences, University of Canterbury, Christchurch 8041, New Zealand 
First page
639
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
2072666X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2544899251
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.