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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Psoriasis is a skin disease that is accompanied by oxidative stress resulting in modification of cell components, including proteins. Therefore, we investigated the relationship between the intensity of oxidative stress and the expression and activity of the proteasomal system as well as autophagy, responsible for the degradation of oxidatively modified proteins in the blood cells of patients with psoriasis. Our results showed that the caspase-like, trypsin-like, and chymotrypsin-like activity of the 20S proteasome in lymphocytes, erythrocytes, and granulocytes was lower, while the expression of constitutive proteasome and immunoproteasome subunits in lymphocytes was increased cells of psoriatic patients compared to healthy subjects. Conversely, the expression of constitutive subunits in erythrocytes, and both constitutive and immunoproteasomal subunits in granulocytes were reduced. However, a significant increase in the autophagy flux (assessed using LC3BII/LC3BI ratio) independent of the AKT pathway was observed. The levels of 4-HNE, 4-HNE-protein adducts, and proteins carbonyl groups were significantly higher in the blood cells of psoriatic patients. The decreased activity of the 20S proteasome together with the increased autophagy and the significantly increased level of proteins carbonyl groups and 4-HNE-protein adducts indicate a proteostatic imbalance in the blood cells of patients with psoriasis.

Details

Title
Reduced Proteasome Activity and Enhanced Autophagy in Blood Cells of Psoriatic Patients
Author
Karabowicz, Piotr 1 ; Wroński, Adam 2 ; Ostrowska, Halina 3 ; Waeg, Georg 4 ; Zarkovic, Neven 5 ; Skrzydlewska, Elżbieta 1   VIAFID ORCID Logo 

 Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland; [email protected] 
 Dermatological Specialized Center “DERMAL” NZOZ in Bialystok, 15-453 Bialystok, Poland; [email protected] 
 Department of Biology, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland; [email protected] 
 Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria; [email protected] 
 LabOS, Laboratory for Oxidative Stress, Rudjer Boskovic Institute, Bijenicka 54, HR-1000 Zagreb, Croatia; [email protected] 
First page
7608
Publication year
2020
Publication date
2020
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2548685969
Copyright
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.