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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Endothelization of the luminal surface of vascular grafts is required for their long-term functioning. Here, we have cultivated human endothelial cells (HUVEC) on different 3D matrices to assess cell proliferation, gene expression and select the best substrate for endothelization. 3D matrices were produced by electrospinning from solutions of poly(D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL), and blends of PCL with gelatin (Gl) in hexafluoroisopropanol. Structure and surface properties of 3D matrices were characterized by SEM, AFM, and sessile drop analysis. Cell adhesion, viability, and proliferation were studied by SEM, Alamar Blue staining, and 5-ethynyl-2’-deoxyuridine (EdU) assay. Gene expression profiling was done on an Illumina HiSeq 2500 platform. Obtained data indicated that 3D matrices produced from PCL with Gl and treated with glutaraldehyde provide the most suitable support for HUVEC adhesion and proliferation. Transcriptome sequencing has demonstrated a minimal difference of gene expression profile in HUVEC cultivated on the surface of these matrices as compared to tissue culture plastic, thus confirming these matrices as the best support for endothelization.

Details

Title
General Study and Gene Expression Profiling of Endotheliocytes Cultivated on Electrospun Materials
Author
Stepanova, Alena O 1   VIAFID ORCID Logo  ; Laktionov, Petr P 2 ; Cherepanova, Anna V 1 ; Chernonosova, Vera S 1   VIAFID ORCID Logo  ; Georgiy Yu Shevelev 3 ; Zaporozhchenko, Ivan A 1 ; Karaskov, Alexander M 4 ; Laktionov, Pavel P 1 

 Laboratory of Biomedical Technologies, Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Rechkunovskaya str. 15, 630055 Novosibirsk, Russia; [email protected] (A.V.C.); [email protected] (V.S.C.); [email protected] (I.A.Z.); [email protected] (A.M.K.); [email protected] (P.P.L.); Laboratory of Molecular Medicine, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), Lavrentiev ave. 8, 630090 Novosibirsk, Russia 
 Department of the Regulation of Genetic Processes, Laboratory of Genomics, Institute of Molecular and Cell Biology, Siberian Branch of the Russian Academy of Sciences (IMCB SB RAS), Lavrentiev ave. 8/2, 630090 Novosibirsk, Russia; [email protected]; Department of Natural Sciences, Epigenetics Laboratory, Novosibirsk State University, Pirogova str. 2, 630090 Novosibirsk, Russia 
 Laboratory of Biomedical Chemistry, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), Lavrentiev ave. 8, 630090 Novosibirsk, Russia; [email protected] 
 Laboratory of Biomedical Technologies, Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Rechkunovskaya str. 15, 630055 Novosibirsk, Russia; [email protected] (A.V.C.); [email protected] (V.S.C.); [email protected] (I.A.Z.); [email protected] (A.M.K.); [email protected] (P.P.L.) 
First page
4082
Publication year
2019
Publication date
2019
Publisher
MDPI AG
e-ISSN
19961944
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2548745911
Copyright
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.