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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

This paper presents an enzyme biocatalytic method for grafting lignin (grafting bioprocess) with aniline, leading to an amino-derivatized polymeric product with modified properties (e.g., conductivity, acidity/basicity, thermostability and amino-functionalization). Peroxidase enzyme was used as a biocatalyst and H2O2 was used as an oxidation reagent, while the oxidative insertion of aniline into the lignin structure followed a radical mechanism specific for the peroxidase enzyme. The grafting bioprocess was tested in different configurations by varying the source of peroxidase, enzyme concentration and type of lignin. Its performance was evaluated in terms of aniline conversion calculated based on UV-vis analysis. The insertion of amine groups was checked by 1H-NMR technique, where NH protons were detected in the range of 5.01–4.99 ppm. The FTIR spectra, collected before and after the grafting bioprocess, gave evidence for the lignin modification. Finally, the abundance of grafted amine groups was correlated with the decrease of the free –OH groups (from 0.030 to 0.009 –OH groups/L for initial and grafted lignin, respectively). Additionally, the grafted lignin was characterized using conductivity measurements, gel permeation chromatography (GPC), thermogravimetric analysis (TGA), temperature-programmed desorption (TPD-NH3/CO2) and scanning electron microscopy (SEM) analyses. The investigated properties of the developed lignopolymer demonstrated its disposability for specific industrial applications of derivatized lignin.

Details

Title
Biocatalytic Strategy for Grafting Natural Lignin with Aniline
Author
Ion, Sabina Gabriela 1 ; Brudiu, Teodor 1 ; Hanganu, Anamaria 2   VIAFID ORCID Logo  ; Munteanu, Florentina 3   VIAFID ORCID Logo  ; Madalin Enache 4   VIAFID ORCID Logo  ; Gabriel-Mihai, Maria 4 ; Tudorache, Madalina 1 ; Parvulescu, Vasile 1 

 Department of Organic Chemistry, Biochemistry and Catalysis, University of Bucharest, Soseaua Panduri 90, sector 5, 050663 Bucharest, Romania; [email protected] (S.G.I.); [email protected] (T.B.); [email protected] (V.P.) 
 Institute of Organic Chemistry C. D. Nenitescu of Romanian Academy, 202B Spl. Independentei, 060023 Bucharest, Romania; [email protected] 
 Department of Technical and Natural Sciences, Aurel Vlaicu University of Arad, Bd. Revolutiei 77, 310130 Arad, Romania; [email protected] 
 Institute of Biology Bucharest of the Romanian Academy, Splaiul Independentei 296, 060031 Bucharest, Romania; [email protected] (M.E.); [email protected] (G.-M.M.) 
First page
4921
Publication year
2020
Publication date
2020
Publisher
MDPI AG
e-ISSN
14203049
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2550236132
Copyright
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.