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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

A rapid, selective and sensitive method for the detection of caffeine in tea infusion and tea beverages are proposed by using 3,5-diaminobenzoic acid as a fluorescent probe. The 3,5-diaminobenzoic acid emits strong fluorescence around 410 nm under the excitation of light at 280 nm. Both the molecular electrostatic potential analysis and fluorescent lifetime measurement proved that the existence of caffeine can quench the fluorescence of 3,5-diaminobenzoic acid. Under the optimal experimental parameters, the 3,5-diaminobenzoic acid was used as a fluorescent probe to detect the caffeine aqueous solution. There exists a good linear relationship between the fluorescence quenching of the fluorescent probe and the concentration of caffeine in the range of 0.1–100 μM, with recovery within 96.0 to 106.2%, while the limit of detection of caffeine is 0.03 μM. This method shows a high selectivity for caffeine. The caffeine content in different tea infusions and tea beverages has been determined and compared with the results from HPLC measurement.

Details

Title
Fluorescence Sensing of Caffeine in Tea Beverages with 3,5-diaminobenzoic Acid
Author
Du, Chenxu 1   VIAFID ORCID Logo  ; Ma, Chaoqun 2 ; Jiao Gu 2 ; Li, Lei 1 ; Chen, Guoqing 2 

 School of Science, Jiangnan University, Wuxi 214122, China; [email protected] (C.D.); [email protected] (C.M.); [email protected] (J.G.); [email protected] (L.L.) 
 School of Science, Jiangnan University, Wuxi 214122, China; [email protected] (C.D.); [email protected] (C.M.); [email protected] (J.G.); [email protected] (L.L.); Jiangsu Provincial Research Center of Light Industrial Optoelectronic Engineering and Technology, Wuxi 214122, China 
First page
819
Publication year
2020
Publication date
2020
Publisher
MDPI AG
e-ISSN
14248220
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2550378697
Copyright
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.