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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Simple Summary

Olives are cultivated mostly in the Mediterranean as well as in Asia Minor, Korea, Japan, and China. Olive oil is currently used as a food ingredient in human diet, and its consumption is gradually expanding in various countries. Therefore, olive cultivation and oil extraction produce a significant amount of byproducts; providing these byproducts as feed to livestock has been attempted for a long period. Economic, environmental, and nutritional considerations make the use of olive byproducts efficient and cost-effective as feed for ruminants. Among the olive byproducts, olive leaves (OLs) contain higher levels of polyphenols than olive fruits, and have a very high feed value. In this study, it was confirmed that methane production decreased during 12 h of in vitro fermentation, and the number of fat-utilizing microorganisms increased in the 5% OLs group. OLs were found to show antioxidant and antimicrobial activity. Moreover, the proportion of cellulose-degrading bacteria, Fibrobacter succinogenes, Ruminococcus albus, and Ruminococcus flavefaciens increased in the 5% OLs group at 12 h and decreased at 24 h. Olive leaves are believed to be very useful as feed additives and supplements for ruminants.

Abstract

We evaluated whether olive leaves (OLs) are effective as feed additives and supplements for ruminants and the potential methane reduction effects during in vitro fermentation. Two Hanwoo cows (460 ± 20 kg) equipped with cannula were fed Timothy hay and corn-based feed 3% of the body weight at a ratio of 6:4 (8:30 a.m. and 5:00 p.m.). Ruminal fluid from the cows was collected and mixed before morning feeding. In vitro batch fermentation was monitored after 12 and 24 h of incubation at 39 °C, and OLs were used as supplements to achieve the concentration of 5% in the basal diet. At 12 h of fermentation, methane production decreased in the 5% OLs group compared to that in the control group, but not at 24 h. The proportion of cellulose-degrading bacteria, Fibrobacter succinogenes, Ruminococcus albus, and Ruminococcus flavefaciens, tended to increase in the 5% OLs group at 12 h. The amount of ammonia produced was the same as the polymerase chain reaction result for Prevotella ruminicola. At 12 h, the proportion of Prevotella ruminicola was significantly higher in the 5% OLs group. OLs may be used incorporated with protein byproducts or other methane-reducing agents in animal feed.

Details

Title
Effects of Olive (Olea europaea L.) Leaves with Antioxidant and Antimicrobial Activities on In Vitro Ruminal Fermentation and Methane Emission
Author
Lee, Shin Ja 1   VIAFID ORCID Logo  ; Kim, Hyun Sang 2   VIAFID ORCID Logo  ; Eom, Jun Sik 2 ; You Young Choi 2 ; Jo, Seong Uk 2 ; Gyo Moon Chu 3 ; Lee, Yookyung 4 ; Seo, Jakyeom 5 ; Kim, Kyoung Hoon 6 ; Sung Sill Lee 7   VIAFID ORCID Logo 

 Institute of Agriculture and Life Science & University-Centered Labs, Gyeongsang National University, Jinju-si 52828, Gyeongsangnam-do, Korea; [email protected] 
 Division of Applied Life Science (BK21), Gyeongsang National University, Jinju-si 52828, Gyeongsangnam-do, Korea; [email protected] (H.S.K.); [email protected] (J.S.E.); [email protected] (Y.Y.C.); [email protected] (S.U.J.) 
 Nonghyupfeed INC. 337, Uam-ro, Nam-gu, Busan 48475, Korea; [email protected] 
 Animal Nutrition and Physiology Team, National Institute of Animal Science, RDA, Jeonju-si 55365, Jeonrabuk-do, Korea; [email protected] 
 Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea; [email protected] 
 Department of International Agricultural Technology, Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Gangwon-do, Korea; [email protected]; Department of Ecofriendly Livestock Science, Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Gangwon-do, Korea 
 Institute of Agriculture and Life Science & University-Centered Labs, Gyeongsang National University, Jinju-si 52828, Gyeongsangnam-do, Korea; [email protected]; Division of Applied Life Science (BK21), Gyeongsang National University, Jinju-si 52828, Gyeongsangnam-do, Korea; [email protected] (H.S.K.); [email protected] (J.S.E.); [email protected] (Y.Y.C.); [email protected] (S.U.J.) 
First page
2008
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
20762615
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2554361851
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.