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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Age-related macular degeneration (AMD) causes the degeneration of photoreceptors and retinal cells leading to vision loss in older subjects. Among possible exogenous risk factors, it has been recently proposed that long-term exposure to blue light could aggravate the course of AMD. In the search for therapeutic options, plasma rich in growth factors (PRGF) has been shown to enhance cell antioxidant pathways and protect photoreceptors against the harm produced by blue light, although its mechanism of action remains unknown. One possible mechanism, autophagy, is one of the most conservative cell renewal systems used in eukaryotes to destroy cellular components that have been damaged by some kind of insult. The oxidative stress of exposure to blue light is known to induce cell autophagy. In this study, we examined the combined effects on autophagy of blue light and PRGF in a retinal cell line, ARPE19. In response to treatment with both PRGF and blue light, we detected the modulated expression of autophagy markers such as NF-kB, p62/sqstm1, Atg5, LC3 and Beclin1, and inflammatory markers such as IL1B and IL18. Our findings suggest that PRGF promotes cell autophagy in response to exposure to blue light.

Details

Title
Plasma Rich in Growth Factors Promotes Autophagy in ARPE19 Cells in Response to Oxidative Stress Induced by Blue Light
Author
Suárez-Barrio, Carlota 1   VIAFID ORCID Logo  ; Susana del Olmo-Aguado 2   VIAFID ORCID Logo  ; García-Pérez, Eva 1   VIAFID ORCID Logo  ; Fernández-Vega-Cueto, Luis 2 ; Andrés Fernández-Vega Cueto 2   VIAFID ORCID Logo  ; Baamonde-Arbaiza, Begoña 2 ; Fernández-Vega, Luis 2 ; Merayo-Lloves, Jesús 2 

 Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica & Universidad de Oviedo, 33012 Oviedo, Spain; [email protected] (C.S.-B.); [email protected] (E.G.-P.); [email protected] (L.F.-V.-C.); [email protected] (A.F.-V.C.); [email protected] (B.B.-A.); [email protected] (L.F.-V.); [email protected] (J.M.-L.) 
 Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica & Universidad de Oviedo, 33012 Oviedo, Spain; [email protected] (C.S.-B.); [email protected] (E.G.-P.); [email protected] (L.F.-V.-C.); [email protected] (A.F.-V.C.); [email protected] (B.B.-A.); [email protected] (L.F.-V.); [email protected] (J.M.-L.); Instituto de Investigación Sanitaria del Principado de Asturias, Avenida de Roma s/n, 33011 Oviedo, Spain 
First page
954
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
2218273X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2554441287
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.