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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Gelatin methacryloyl (GelMA) hydrogel is a photopolymerizable biomaterial widely used for three-dimensional (3D) cell culture due to its high biocompatibility. However, the drawback of GelMA hydrogel is its poor mechanical properties, which may compromise the feasibility of biofabrication techniques. In this study, a cell-laden GelMA composite hydrogel with a combination incorporating silanized hydroxyapatite (Si-HAp) and a simple and harmless visible light crosslinking system for this hydrogel were developed. The incorporation of Si-HAp into the GelMA hydrogel enhanced the mechanical properties of the composite hydrogel. Moreover, the composite hydrogel exhibited low cytotoxicity and promoted the osteogenic gene expression of embedded MG63 cells and Human bone marrow mesenchymal stem cells (hBMSCs). We also established a maskless lithographic method to fabricate a defined 3D structure under visible light by using a digital light processing projector, and the incorporation of Si-HAp increased the resolution of photolithographic hydrogels. The GelMA-Si-HAp composite hydrogel system can serve as an effective biomaterial in bone regeneration.

Details

Title
Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light Projection
Author
Su, Jimmy Jiun-Ming 1 ; Lin, Chih-Hsin 2 ; Chen, Hsuan 3   VIAFID ORCID Logo  ; Lee, Shyh-Yuan 4   VIAFID ORCID Logo  ; Yuan-Min, Lin 4   VIAFID ORCID Logo 

 Institute of Oral Biology, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; [email protected] 
 Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 110, Taiwan; [email protected] 
 Department of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; [email protected] (H.C.); [email protected] (S.-Y.L.) 
 Department of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; [email protected] (H.C.); [email protected] (S.-Y.L.); Department of Stomatology, Taipei Veterans Hospital, Taipei 11221, Taiwan 
First page
2354
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
20734360
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2554778859
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.