The effect of tomato juice (Lycopersicon esculentum) as natural antioxidant to fertilization rate spermatozoa of kancra fish (Tor soro) 24 hours postcryopreservation

Abstract

Kancra fish (Tor soro) is one of endemic freshwater fish which is originated from Sumatera island. Nowdays, the population of kancra fish is declining because of over fishing in the habitat. Therefore, it is necessary to preserve kancra fish by doing spermatozoa cryopreservation. The objective of present study is to evaluated the effect of tomato juice on fertilization rate 24 hour after spermatozoa cryopreservation. The study used a completely randomized design with four treatments and six replications. Four treatments consisted of 0% tomato juice + 10% DMSO (0% SBt); 10% tomato juice + 10% DMSO (10% SBt); 20% tomato juice + 10% DMSO (20% SBt); and 30% tomato juice + 10% DMSO (SBt 30%). Data was analysed by One Way Analyses of Variance test followed by Tukey test. The results showed that there was the tomato juice effect (P<0.05) on the fertilization rate after spermatozoa was deep frozen in liquid nitrogen (LN) for 24 hours has significant difference (P <0.05) in the average value of the percentage of fertilization rate. The results showed that the 10% of tomato juice was optimum concentration that showed the highest fertilization rate (81.25 ± 6.07%).