Abstract

Characteristic of cytochrome C oxidase subunit I (COI) gene in giant clam from Wakatobi National Park Waters was studied to detect the specific nucleotide and show the relationship among species of giant clams. Sample collection was conducted around Wangi-wangi, Kaledupa, and Tomia waters. Genomic DNA was extracted from clam mantle tissue of two generas, and amplified by PCR method. We obtained nucleotide size of 522 bp for genus Tridacna (T. crocea, T. maxima) and 708 bp for genus Hippopus (H. hippopus). There were 52 specific nucleotide for the clams (included T. gigas and T. squamosa from GenBank data). T. crocea, T. gigas, T. squamosa, T. maxima, and H. hippopus had 2, 3, 9, 2, and 36 sites, respectively. Reconstruction phylogenetic tree using Neighbour Joining of 476 bp COI gene sequences showed that all species of giant clams were monophyletic.

Details

Title
Characteristics of cytochrome C oxidase subunit I gene in giant clam from Wakatobi National Park Waters, Indonesia
Author
Findra, M N 1 ; Setyobudiandi, I 2 ; Solihin, D D 3 ; Butet, N A 2 

 Department of Aquatic Resource Management, Faculty of Fisheries and Marine Science, Halu Oleo University, Kendari, Indonesia 
 Department of Aquatic Resource Management, Faculty of Fisheries and Marine Science, IPB University, Bogor, Indonesia 
 Department of Biology, Faculty of Mathematics and Science, IPB University, Bogor, Indonesia 
Publication year
2020
Publication date
Oct 2020
Publisher
IOP Publishing
ISSN
17551307
e-ISSN
17551315
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2556405184
Copyright
© 2020. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.