Activation of the body’s natural defenses reserve of mice treated with various physico-chemical agents

Abstract

The purpose of this work is to reveal the activation of natural defenses reserve in mice after treatment with different physico-chemical agents in vivo using previously developed technology of adaptive response induction. Physical agents were represented by X-rays, carbon ions, infrared light, He-Ne laser light, famine and chemical agents – by immunomodulator CaCl₂ and anti-inflammatory drug ibuprofen. The following tasks were set: assessment of cytogenetic damage using a micronucleus test, the weight index of lymphoid organs (thymus and spleen) and the level of ROS production in whole blood through the method of luminol-dependent zymosan-induced chemiluminescence. SHK mice were irradiated according to the scheme of adaptive response. Analysis of data on the number of cytogenetic damage in bone marrow showed that pretreatment of the animals with all investigated agents and subsequent exposure to X-rays or carbon ions at a dose of 1.5 Gy has led to a decrease in radiosensitivity compared to the nontreated animals. Similar results were observed when analyzing weight index of lymphoid organs. Determination of level of ROS production has shown that the activation index calculated according to the relation of induced to spontaneous light area, was significantly higher in all groups of mice, indicating activation of the natural defenses reserve as compared to the group exposed only at a dose of 1.5 Gy. The obtained results confirm the assumption of revealing activation of the natural defense of the organism with the help of the adaptive response induction technology.