It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
It is known that nanosized liposomes from vegetable and animal phospholipids promote survival and functional adequacy of animal cells in the course of cryopreservation. It is also known that albumine and some polysaccharides have membrane-protective properties. In this paper we investigated whether there is a synergism of cryoprotective action between soybean liposomes and macromolecular compounds: albumine, sodium alginate, methylcellulose, carageenan and hyaluronic acid. To determine the cryoprotective effect we carried out cryopreservation of bull semen using a standard procedure in the presence of the substances under investigation. Albumine and sodium alginate demonstrated the most considerable cryoprotective effect in the presence of liposomes. In addition we determined dimensional characteristics of nanoparticles in a liposome suspension without and with sodium alginate and studied the influence of autoclave treatment on the nanoparticle size and cryoprotective effect of the complex. Autoclaving caused enlargement of a small-sized fraction of nanoparticles, supposedly, sodium alginate aggregates. Besides, the autoclaving did not demonstrate any influence on the cryoprotective efficiency of the mixture.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 Institute of Cell Biophysics, Russian Academy of Sciences, 3, Institutskaya st., Pushchino, 142290, Russia
2 Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 3, Institutskaya st., Pushchino, 142290, Russia
3 OJSC Head Center for the Reproduction of Farm Animals, Moscow Region, Russia
4 Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 3, Institutskaya st., Pushchino, 142290, Russia; Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-3, Moskow, Russia
5 IAM RAS, 7, Leningradsky prt, 125040, Moscow, Russia
6 National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of Russian Federation, 4 Oparin str, 117997, Moscow, Russia