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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Simple Summary

In human lymphomas, it has been shown that the macrophages present in the tumor have an influence on their behavior; however, studies on this topic are scarce in veterinary medicine. The aim of this work was to determine the relationship between the amount and type of macrophage infiltrates with histological grade and immunophenotype in cases of canine lymphoma. Samples from the lymph nodes of 25 dogs with lymphoma were analyzed. Immunohistochemistry was used to determine the tumor immunophenotype (CD3 and CD20 antibodies) and the macrophage characterization (Iba1, CD163, iNOS and MAC387 antibodies). Results showed that the highest number of macrophages were found in high-grade and B-cell lymphomas; the latter also presented the greatest amount of M1 and M2 macrophages. High-grade lymphomas showed a greater number of M2 and recently recruited macrophages that were most abundant in T than in B-cell lymphomas. In conclusion, the number and type of macrophages present in canine lymphoma are related to the immunophenotype and the grade. In those with a high grade, macrophages are actively recruited and show a predominant M2 phenotype, which has been associated with a protumoral activity.

Abstract

Macrophages have been confirmed to play a significant role in the behavior of human lymphomas, albeit no consistent data are so far available in canine lymphomas. The present study characterizes the macrophages present in cases of canine nodal lymphoma and their relationship with the histological grade and the immunophenotype. Samples from the lymph nodes of 25 dogs diagnosed with lymphoma were selected. Immunohistochemistry was used to determine the tumor immunophenotype (CD3 and CD20 antibodies) and macrophage characterization (Iba1, MAC387, CD204, CD163 and iNOS antibodies). Macrophage counting was performed in 10 randomly selected, high-power fields per sample. Generalized linear models with Poisson distribution were used for statistical analysis. A significantly greater number of macrophages (Iba1+) were detected in high-grade and B-cell lymphomas. The highest amount of both M1 (iNOS+) and M2 (CD204+ and CD163+) subtypes were observed in B-cell lymphomas. High-grade lymphomas showed a greater number of CD204+ and CD163+ cells and recently recruited MAC387+ macrophages. The latter were most abundant in T than in B-cell lymphomas. In conclusion, a significant population of macrophages is present in canine lymphomas, which constitute a heterogeneous population that shows variations in the amount and immunohistochemical profile according to the histological grade and immunophenotype.

Details

Title
Immunohistochemical Characterization of Tumor-Associated Macrophages in Canine Lymphomas
Author
Vázquez, Sergio 1   VIAFID ORCID Logo  ; Vallejo, Raquel 1   VIAFID ORCID Logo  ; Espinosa, José 1   VIAFID ORCID Logo  ; Arteche, Noive 1 ; Vega, José A 2   VIAFID ORCID Logo  ; Pérez, Valentín 1   VIAFID ORCID Logo 

 Departamento de Sanidad Animal, Universidad de León, 24007 León, Spain; [email protected] (R.V.); [email protected] (J.E.); [email protected] (N.A.); [email protected] (V.P.) 
 Grupo SINPOS, Departamento de Morfología y Biología Celular, Universidad de Oviedo, 33006 Oviedo, Spain; [email protected]; Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Providencia 7500912, Santiago de Chile, Chile 
First page
2301
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
20762615
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2564512780
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.