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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

The kidney is a main site of erythropoietin production in the body. We developed a new method for the detection of Epo protein by deglycosylation-coupled Western blotting. Detection of deglycosylated Epo enables the examination of small changes in Epo production. Using this method, we investigated the effects of angiotensin II (ATII) on Epo production in the kidney. ATII stimulated the plasma Epo concentration; Epo, HIF2α, and PHD2 mRNA expression in nephron segments in the renal cortex and outer medulla; and Epo protein expression in the renal cortex. In situ hybridization and immunohistochemistry revealed that ATII stimulates Epo mRNA and protein expression not only in proximal tubules but also in collecting ducts, especially in intercalated cells. These data support the regulation of Epo production in the kidney by the renin–angiotensin–aldosterone system (RAS).

Details

Title
Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver
Author
Yasuoka, Yukiko 1 ; Izumi, Yuichiro 2   VIAFID ORCID Logo  ; Fukuyama, Takashi 3 ; Inoue, Hideki 2 ; Oshima, Tomomi 1 ; Yamazaki, Taiga 3   VIAFID ORCID Logo  ; Uematsu, Takayuki 3   VIAFID ORCID Logo  ; Kobayashi, Noritada 3 ; Shimada, Yoshitaka 4 ; Nagaba, Yasushi 4   VIAFID ORCID Logo  ; Mukoyama, Masashi 2 ; Sato, Yuichi 5   VIAFID ORCID Logo  ; Sands, Jeff M 6   VIAFID ORCID Logo  ; Kawahara, Katsumasa 1 ; Nonoguchi, Hiroshi 4   VIAFID ORCID Logo 

 Department of Physiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0374, Kanagawa, Japan; [email protected] (Y.Y.); [email protected] (T.O.); [email protected] (K.K.) 
 Department of Nephrology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Kumamoto, Japan; [email protected] (Y.I.); [email protected] (H.I.); [email protected] (M.M.) 
 Division of Biomedical Research, Kitasato University Medical Center, 6-100 Arai, Kitamoto 364-8501, Saitama, Japan; [email protected] (T.F.); [email protected] (T.Y.); [email protected] (T.U.); [email protected] (N.K.) 
 Division of Internal Medicine, Kitasato University Medical Center, 6-100 Arai, Kitamoto 364-8501, Saitama, Japan; [email protected] (Y.S.); [email protected] (Y.N.) 
 Department of Molecular Diagnostics, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0373, Kanagawa, Japan; [email protected] 
 Renal Division, Department of Medicine, Emory University School of Medicine, 1639 Pierce Drive, WMB Room 3313, Atlanta, GA 30322, USA; [email protected] 
First page
5399
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
14203049
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2571437645
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.