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Abstract
Segmented capillaries comprising two segments with different surface charges of polymer layers are prepared. One segment with a neutral surface charge is responsible for separation and another one with negatively charged surface provides the electro-osmotic flow adjustment. The average electro-osmotic flow rate is found to correlate with the length of negatively charged segment. A mathematical model is suggested to describe electromigration performances of segmented capillaries depending on the segments length ratio also taking into account the stationary phase/coating with definite surface properties (ξ—potential) or electroosmotic flow rate.
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Details
1 Institute for Analytical Instrumentation, RAS, St. Petersburg, Russia
2 St. Petersburg State Institute of Technology (Technical University), Russia
3 St. Petersburg State University of Economics, Russia