Identification of Microscopic Fungus in Gill of Skipjact Tuna (Katsuwonus pelamis L.) at Traditional Markets in Singaraja - Bali

Abstract

Skipjack tuna is a medium-sized fish from family of Scombridae (tuna) which is currently widely sold in traditional markets in Singaraja - Bali. The freshness of the fish in the markets is strongly influenced by microorganisms especially microscopic fungus that contaninated from market environment. The purposes of this research were determined the number of microscopic polyculture colonies on skipjack tuna that sold in traditional markets in Singaraja - Bali, and microscopic fungus found in the gill of skipjack tuna (Katsuwonus pelamis L.). This research is used a qualitative approach in the form of descriptive explorative by isolating and identifying microscopic fungi. The subjects of this research were skipjack fish from traders in three traditional markets spread in Singaraja – Bali, such as Anyar Market, Banyuasri Market and Banyuning Market. While the object of this research was gill of skipjack tuna. As much as one gram of skipjack tuna was introduced into the sterile distillation as the first dilution and was carried out until 10^-6 dilution to calculate the number of microscopic policulture colonies per dilution. Furthermore, microscopic fungus suspected to have different morphological characteristics were isolated to find pure isolates. Identification of each fungus as macroscopically and microscopically was performed after the purification. The results of this research indicated that the average of total microscopic polyculture colony in Anyar market amounts to 5.3 x 10⁵ cfu/ml; in the Banyuasri market of 2.2 x 10⁵ cfu/ml and Banyuning market at 6.4 x 10⁴ cfu / ml. There were 8 genera of microscopic fungus found in skipjack tuna in the three traditional markets in Singaraja city, namely Aspergillus, Penicillium, Rhizopus, Mucor, Trichoderma, Culvularia, Verticillium and Cladosporium. All the fungus that found as contaninant from the markets environment.