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Abstract
A scanning ion-conductance microscope was designed on the basis of scanning probe microscope NanoTutor. The optimal parameters of nanopipettes fabrication were found according to scanning electron microscopy diagnostics, current-distance I (Z) and current-voltage characteristics. A comparison of images of test objects, including biological samples, was carried out in the modes of optical microscopy, atomic force microscopy and scanning ion-conductance microscopy. Sphere-shaped nanopippettes probes were developed and tested to increase the stability of pipettes, reduce invasiveness and improve image quality of atomic force microscopy in tapping mode. The efficiency of sphere-shaped nanopippettes is shown.
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Details
1 Department of nanophotonics and metamaterials, ITMO University, St. Petersburg 197101, Russia; Laboratory of scanning probe microscopy and spectroscopy, Institute for Analytical Instrumentation RAS, St. Petersburg 198095, Russia
2 Laboratory of scanning probe microscopy and spectroscopy, Institute for Analytical Instrumentation RAS, St. Petersburg 198095, Russia
3 Laboratory of Ionic Mechanisms of Cell Signaling, Institute of Cytology RAS, St. Petersburg 194064, Russia
4 Department of nanophotonics and metamaterials, ITMO University, St. Petersburg 197101, Russia; Laboratory of renewable energy sources, St Petersburg Academic University RAS, 194021 Khlopina, 8/3, Saint-Petersburg, Russian Federation