Epigallocatechin-3-gallate inhibits paclitaxel-induced apoptosis through the alteration of microRNA expression in human dermal papilla cells

Abstract

Background

Paclitaxel well known as anti-cancer drug has been shown to cause alopecia in chemotherapy. The paclitaxel chemotherapy-mediated alopecia is induced by apoptotic damage in human dermal papilla (HDP) cells. Epigallocatechin-3-gallate (EGCG) inhibits apoptosis against anti-cancer drug such as cisplatin. EGCG, one of the green tea extract ingredients, has been reported to enhance cell viability and to inhibit apoptosis. However, it is unclear that EGCG enhances cell viability and inhibits apoptosis against paclitaxel-induced apoptotic damage in HDP cells.

Methods

We show cell viability, cell cycle, and microRNA (miRNA) expression in EGCG-mediated rescue cell to paclitaxel-mediated cell death and growth arrest.

Results

EGCG promotes cell survival and cell death inhibitory effects and alteration of miRNA expression in paclitaxel-exposed HDP cells were investigated. Firstly, paclitaxel increases apoptosis and EGCG promotes cell survival and represses paclitaxel-induced apoptosis in a dose-dependent manner. Fluorescence-activated cell sorting (FACS) analysis showed that EGCG protects apoptosis in paclitaxel-exposed HDP cells. miRNA microarray analysis was performed and 48 miRNAs changed by EGCG in paclitaxel-exposed HDP cells were identified. In gene ontology analysis in silico, miRNAs regulate apoptosis and cell proliferation-regulated genes, such as BCL2L1, BCL2L2, BBC3, and MDM2. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, miRNAs are related to mitogen-activated protein kinase (MAPK) signaling pathway and Wnt signaling pathway, which regulate apoptosis and cell proliferation.

Conclusions

EGCG inhibits apoptosis through regulating miRNA expression related to apoptosis and cell proliferation in paclitaxel-treated HDP cells.

Background

Paclitaxel, taxane-based anticancer drugs, purified in bark of Taxus brevifolia regulates microtubule dynamics by binding with β-tubulin (Manfredi and Horwitz 1984; Amos and Löwe 1999). Paclitaxel-mediated regulation repressed mitotic activity and then induces apoptosis. Thus, it is generally used in therapy of metastatic breast cancer, ovarian cancer, and non-small cell lung cancer (Khanna et al. 2015; Chen et al. 2011; Jordan and Wilson 2004).

In anticancer chemotherapy, the risk of potential side effects is one of the important features (Macdonald et al. 2015; Balagula et al. 2011). In the case of paclitaxel, a known side effects include leukopenia, thrombocytopenia, anemia, muscle pain, and hair loss symptoms. Especially, hair loss is the major side effects of paclitaxel (Ozcelik et al. 2010; Shapiro and Recht 2001). Paclitaxel-mediated hair loss is resulted by apoptotic damage of dermal papilla cells (Chen et al. 2011; McElwee et al. 2003; Yang and Cotsarelis 2010). However, it has not been identified the detailed mechanism of paclitaxel-mediated apoptotic damage of dermal papilla cells.

Hair is grown by a repetitive cycle consisted of anagen, catagen and telogen, which is regulated by HDP cells in normal and abnormal condition, such as hair loss (Inui et al. 2003; Stenn and Paus 2001). Since the HDP cells can significantly affect the hair growth, HDP cell-regulating factor is implicated in therapy of hair loss (Kwon et al. 2007; Aljuffali et al. 2015).

Recently, miRNA is studied as a factor affecting the function of the dermal papilla cells. miRNA is a non-coding small nucleotide consisted of 18–24 nucleotides and interfere translation of target genes by binding to a 3′-untranslated region (UTR) of the mRNA in the gene expression process (Bartel 2004; Bartel 2009; Carrington and Ambros 2003). miRNA is known to play a role in apoptosis, cell proliferation, and differentiation and recently has been reported that miR-31 is highly expressed in hair growth phase, which regulated the expression of Krt16, Krt17, Dlx3, and Fgf10. In addition, miR-24 is implicated in hair follicle formation through regulating known as stemness regulator of human keratinocytes role (Wan et al. 2011; Mardaryev et al. 2010; Amelio et al. 2013).

EGCG, a major compound of green tea that contained polyphenols, is effective in anti-cancer and antioxidant (Hsu 2005; Wang and Bachrach 2002; Katiyar and Elmets 2001). In addition, EGCG induced cell growth and is prevented against UV-mediated cell death in keratinocytes and HDP cells, which regulate hair growth (Kwon et al. 2007; Yang and Landau 2000; Katiyar et al. 1995; Chung et al. 2003).

In our study, we show protection activity of EGCG to paclitaxel-induced HDP cell death. Moreover, we demonstrate a cellular signaling mechanism of EGCG-mediated paclitaxel protection mechanism through analyzing alteration miRNA expression profile.

Methods

Cell culture and materials

HDP cells were maintained using Dulbecco’s modified Eagle’s medium (DMEM; Hyclone, Logan, UT, USA) with 1% penicillin/streptomycin (10,000 units/mL penicillin G sodium, 10,000 μg/mL streptomycin; Gibco-BRL/Invitrogen Life Technologies, Gaithersburg, MD, USA) and 10% fetal bovine serum (FBS; Hyclone) and incubated at 5% CO₂ at 37 °C. Paclitaxel was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Cell viability assay

HDP cells were seeded in 96-well plates at 2 × 10³ cells/well and then incubated for 24 h. After 24 h, EGCG and paclitaxel was treated with indicated concentrations for 24 h. Water-soluble tetrazolium-1 (WST-1) solution was added and incubated for 20 min. Optical density of each well was measured at 450 nm using a iMark™ microplate reader (Bio-Rad, Hercules, CA, USA). And the value was calibrated by measuring a reference absorbance at 650 nm.

Cell cycle assay

HDP cells were incubated with EGCG and paclitaxel for 24 h. Cells were harvested and washed with phosphate-buffered saline (PBS; sodium chloride 137 mM, phosphate buffer 10 mM, potassium chloride 2.7 mM, all from BioPure (Canada). After washing, cells were fixed by 70% ethanol and then stained with propidium iodide (PI) staining buffer (PI 50 μg/mL, RNase 0.1 mg/mL, 0.05% Triton X-100). Fluorescence intensity was measured by FACS Calibur (BD Biosciences, San Jose, CA, USA). And Sub-G1, G0/G1, S, and G2/M were measured by Cell Quest software (BD Biosciences).

miRNA microarray

To analyze miRNA expression profile, HDP cells were seeded and treated with EGCG and paclitaxel. After 24 h, total RNA was extracted using TRIzol reagent (Sigma-Aldrich) according to the manufacturer’s instructions. Total RNA was stained with Cy3 using Agilent miRNA Labeling kit (Agilent Technologies, Santa Clara, CA, USA). Labeled RNAs were hybridized using a SurePrint G3 Human v16 miRNA 8x60K microarray (Agilent Technologies) at 65 °C for 20 h. The miRNA expression profile was analyzed using Feature Extraction version 10.7 software (Agilent Technologies) and GeneSpring GX software, version 11.5 (Agilent Technologies).

miRNA target and ontology analysis

Target genes were predicted using seed sequence-based miRNA target prediction database, TargetScan (Target scan human 2017) and miRbase (miRbase 2017). Additionally, to categorize hair follicle development, apoptosis, and cell proliferation, gene ontology of predicted target genes of miRNA was analyzed by DAVID Bioinformatics Resources 6.7 (National Institute of Allergy and Infectious Diseases 2017).

Results

Protective effects of EGCG to paclitaxel-mediated growth arrest in HDP cells

To demonstrate protection effects of EGCG in paclitaxel-mediated growth arrest, we co-treated it with paclitaxel and EGCG. As shown in Fig. 1, 5 μM EGCG decreased 1 μM paclitaxel-induced growth arrest (62%) to 84%. Furthermore, in cell cycle analysis, 1 μM paclitaxel induced cell death fraction (sub-G1) in the same condition. However, co-treatment with 10 μM EGCG reduced paclitaxel-mediated cell death in HDP cells (Fig. 2).

EGCG-mediated alteration of miRNA profile in paclitaxel-exposed HDP cells

We showed EGCG-mediated alteration of miRNA profile in paclitaxel-exposed HDP cells. As shown in Fig. 3, EGCG upregulated 20 miRNAs and downregulated 28 miRNAs in paclitaxel-exposed HDP cells. Among 48 miRNAs, miR-3663-3p, miR-1181, miR-3613-3p, miR-1281, and miR-1539 had increased by 293.52-fold, 241.98-fold, 188.91-fold, 177.18-fold, and 169.23-fold, respectively. Additionally, miR-221-5p, miR-374b, miR-590-5p, miR-4306, and miR-500a-5p had decreased by 132.32-fold, 124.96-fold, 122.52-fold, 116.09-fold, and 105.14-fold, respectively (Table 1).

miRNA	Change relative to controls	Direction of regulation	chr.	miRNA	Change relative to controls	Direction of regulation	chr.
hsa-miR-3663-3p	293.53	Up	chr10	hsa-miR-500a-5p	105.14	Down	chrX
hsa-miR-1181	241.98	Up	chr19	hsa-miR-299-3p	102.72	Down	chr14
hsa-miR-3613-3p	188.92	Up	chr13	hsa-miR-140-5p	97.50	Down	chr16
hsa-miR-1281	177.19	Up	chr22	hsa-miR-193a-5p	73.05	Down	chr17
hsa-miR-1539	169.23	Up	chr18	hsa-miR-34a-3p	61.13	Down	chr1
hsa-miR-125a-3p	159.16	Up	chr19	hsa-miR-432-5p	60.05	Down	chr14
hsa-miR-4271	158.48	Up	chr3	hsa-miR-204-5p	56.88	Down	chr9
hsa-miR-550a-5p	156.32	Up	chr7	hsa-miR-485-3p	55.67	Down	chr14
hsa-miR-642b-3p	142.71	Up	chr19	hsa-miR-140-3p	55.23	Down	chr16
hsa-miR-23c	134.49	Up	chrX	hsa-miR-409-5p	54.21	Down	chr14
hsa-let-7f-1-3p	108.08	Up	chr9	hsa-miR-146a-5p	52.69	Down	chr5
hsa-miR-874	96.40	Up	chr5	hsa-miR-423-5p	50.69	Down	chr17
hsa-miR-33b-3p	88.74	Up	chr17	hsa-miR-17-3p	50.49	Down	chr13
hsa-miR-3180-5p	76.65	Up	chr16	hsa-miR-487a	47.24	Down	chr14
hsa-miR-150-3p	65.94	Up	chr19	hsa-miR-450a-5p	47.14	Down	chrX
hsa-miR-129-1-3p	64.80	Up	chr7	hsa-miR-138-2-3p	45.41	Down	chr16
hsa-miR-1249	63.67	Up	chr22	hsa-miR-128	44.19	Down	chr2
hsa-miR-3679-3p	60.74	Up	chr2	hsa-miR-28-5p	43.34	Down	chr3
hsa-miR-767-3p	34.07	Up	chrX	hsa-miR-431-5p	43.33	Down	chr14
hsa-miR-3656	3.60	Up	chr11	hsa-miR-146b-5p	43.22	Down	chr10
hsa-miR-221-5p	132.32	Down	chrX	hsa-miR-4317	38.84	Down	chr18
hsa-miR-374b	124.96	Down	chrX	hsa-miR-324-5p	38.47	Down	chr17
hsa-miR-590-5p	122.52	Down	chr7	hsa-miR-370	37.81	Down	chr14
hsa-miR-4306	116.09	Down	chr13	hsa-miR-16-2-3p	22.41	Down	chr3

1. Chr. chromosome

Bioinformatic analysis of EGCG-regulated miRNAs in paclitaxel-exposed HDP cells

To analyze relation between growth arrest and cell death and EGCG-regulated miRNAs, we predicted target genes of EGCG-regulated miRNAs using TargetScan (Target scan human 2017) and miRbase (miRbase 2017). And then, in Additional file 1: Table S1 and Table S2, we categorized functions of target genes using their functions, apoptosis, cell proliferation, and hair follicle development, using DAVID (National Institute of Allergy and Infectious Diseases 2017). Additionally, to demonstrate relation between target genes of EGCG-regulated miRNAs and identified signal pathways, we analyzed signal pathway using KEGG pathway database. As shown in Additional file 1: Table S3 and Table S4, at in silico analysis, EGCG-upregulated miRNAs regulated various KEGG pathways, such as MAPK signaling pathway, pathways in cancer, neurotrophin signaling pathway, long-term depression, pancreatic cancer, chronic myeloid leukemia, colorectal cancer, ECM-receptor interaction, vibrio cholera infection, glycerolipid metabolism, lysine degradation, cell adhesion molecules (CAMs), glioma, melanoma, endocytosis, focal adhesion, ubiquitin-mediated proteolysis, ErbB signaling pathway, tight junction, Jak-STAT signaling pathway, renal cell carcinoma, adherens junction, Fc gamma R-mediated phagocytosis, epithelial cell signaling in Helicobacter pylori infection, p53 signaling pathway, long-term potentiation, phosphatidylinositol signaling system, non-small cell lung cancer, aldosterone-regulated sodium reabsorption, Wnt signaling pathway, regulation of actin cytoskeleton, axon guidance, TGF-beta signaling pathway, melanogenesis, basal cell carcinoma, arrhythmogenic right ventricular cardiomyopathy (ARVC), pathogenic Escherichia coli infection, circadian rhythm, T cell receptor signaling pathway, B cell receptor signaling pathway, glycosphingolipid biosynthesis, valine, leucine and isoleucine degradation, insulin signaling pathway, Hedgehog signaling pathway, mTOR signaling pathway, RNA degradation, heparan sulfate biosynthesis, one carbon pool by folate, prostate cancer, cell cycle, spliceosome, apoptosis, small cell lung cancer, acute myeloid leukemia, adipocytokine signaling pathway, endometrial cancer, inositol phosphate metabolism, and sphingolipid metabolism in paclitaxel-treated HDP cells, and EGCG-downregulated miRNAs regulated Fc gamma R-mediated phagocytosis, pathways in cancer, MAPK signaling pathway, focal adhesion, regulation of actin cytoskeleton, Wnt signaling pathway, chemokine signaling pathway, axon guidance, TGF-beta signaling pathway, renal cell carcinoma, melanoma, melanogenesis, basal cell carcinoma, ErbB signaling pathway, prostate cancer, Hedgehog signaling pathway, p53 signaling pathway, notch signaling pathway, cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, calcium signaling pathway, T cell receptor signaling pathway, dilated cardiomyopathy, amino sugar and nucleotide sugar metabolism, ubiquitin-mediated proteolysis, pathogenic Escherichia coli infection, small cell lung cancer, valine, leucine and isoleucine degradation, purine metabolism, gap junction, glycerolipid metabolism, Huntington’s disease, N-glycan biosynthesis, fatty acid metabolism, butanoate metabolism, glioma, long-term depression, cysteine and methionine metabolism, neurotrophin signaling pathway, adherens junction, vascular smooth muscle contraction, insulin signaling pathway, progesterone-mediated oocyte maturation, oocyte meiosis, non-small cell lung cancer, chronic myeloid leukemia, dorso-ventral axis formation, mTOR signaling pathway, phosphatidylinositol signaling system, CAMs, endocytosis, splicesome, endometrial cancer, tight junction, chondroitin sulfate biosynthesis, inositol phosphate metabolism, colorectal cancer, GnRH signaling pathway, ECM-receptor interaction, Fc epsilon RI signaling pathway, ABC transporters, neuroactive ligand-receptor interaction, aldosterone-regulated sodium reabsorption, propanoate metabolism, natural killer cell-mediated cytotoxicity, B cell receptor signaling pathway, vascular endothelial growth factor (VEGF) signaling pathway, and pyrimidine metabolism in paclitaxel-treated HDP cells.

Discussion

Therapeutic effect of paclitaxel on tumor cells is reported in many different cancers (Sunters et al. 2003; Millenbaugh et al. 1998; Ettinger 1993). However, paclitaxel also affects other normal cell and then occurs a lot of side effects (Chon et al. 2012). Especially, paclitaxel-induced hair loss, one of the paclitaxel side effects, is caused by the apoptotic damage in HDP cells, one of the key players to form hair follicle and grow hair (Chen et al. 2011). EGCG extracted from green tea has been shown as effective anti-cancer agent and antioxidation agent in normal cells (Hsu 2005; Wang and Bachrach 2002; Hsu et al. 2003). In addition, EGCG is attenuated in cisplatin-mediated HDP cell death (Hsu et al. 2003). Thus, we showed effects of EGCG in paclitaxel-mediated HDP cell death. In this study, to determine regulation of miRNAs by EGCG in paclitaxel-exposed HDP cells, we showed the EGCG-mediated alteration of miRNA profile in HDP cells treated with paclitaxel.

Firstly, we showed that EGCG repressed paclitaxel-mediated growth arrest and cell death in HDP cells (Figs. 1 and 2). In the same condition, we analyzed miRNA profile, since, in recent study, HDP cells regulate cellular signal pathway, such as survival, death, and cell cycle arrest (Kim et al. 2014; Cha et al. 2014). As shown in Figs. 1 and 3, 48 miRNAs have confirmed the EGCG-mediated upregulation or downregulation in paclitaxel-treated HDP cells. In significantly up- and downregulated miRNA, miR-129-1-3p (64.8-fold upregulation) is reported as repressor of PDCD2 which is overexpressed in proliferation of human gastric cancer (Du et al. 2014) and miR-128 (44.19-fold downregulation) was reported to inhibit cell growth, when overexpressed in head and neck squamous cell carcinoma (Hauser et al. 2015). Additionally, EGCG-regulated miRNAs targeted FOXO1, BCL2L1, BCL2L2, PTEN, BBC3, and MDM2 which are the regulator genes against cell growth, proliferation, and cell death. When FOXO1 is overexpressed, cell proliferation and growth is inhibited by upregulating caspase-3 and caspase-9 and inducing G2/M arrest and (Yang et al. 2015). BCL2L1 and BCL2L2, BCL2 family, are pro-oncogene and repress intrinsic pathway-mediated cell death (Denoyelle et al. 2014; Zhang et al. 2015). Other genes, PTEN, BBC3, and MDM2, also rescue cell death and induce proliferation (Fortunato et al. 2014; Zhang et al. 2010).

In KEGG pathway analysis against target genes, we find upregulated miRNA-related 58 KEGG pathways and downregulated miRNA-related 68 KEGG pathways. Among these KEGG pathway, Wnt signaling pathway, pathways in cancer, MAPK signaling pathway, and basal cell carcinoma, adherens junction is highly related with upregulated miRNA. And MAPK signaling pathway, neurotrophin signaling pathway, melanogenesis, axon guidance, Wnt signaling pathway are most related to downregulated miRNAs. MAPK signaling pathway and Wnt signaling pathway related to both up- and downregulated miRNAs are involved in cell survival and apoptosis (Zhang et al. 2010; Shang et al. 2015). Thus, we suggest that EGCG-mediated miRNA expression change regulates cell survival and apoptosis through MAPK signaling pathway and Wnt signaling pathway.

Following our results, we demonstrate that EGCG-regulated alteration of miRNA expression increase proliferation and decrease cell death in paclitaxel-treated HDP cells. Additionally, these results suggest that EGCG reduced paclitaxel-mediated hair loss by alteration of miRNA profile.

Conclusions

This is the first study to address positive effects of EGCG in paclitaxel-mediated hair loss. The study demonstrated that EGCG increases proliferation, decreases cell death by alteration of miRNA profile in HDP cells, and sequentially affects paclitaxel-mediated hair loss.

Abbreviations

ARVC: Arrhythmogenic right ventricular cardiomyopathy

CAMs: Cell adhesion molecules

DMEM: Dulbecco’s modified Eagle’s medium

EGCG: Epigallocatechin-3-gallate

FACS: Fluorescence-activated cell sorting

FBS: Fetal bovine serum

HDP: Human dermal papilla

KEGG: Kyoto Encyclopedia of Genes and Genomes

MAPK: Mitogen-activated protein kinase

miRNA: MicroRNA

PBS: Phosphate-buffered saline

PI: Propidium iodide

UTR: Untranslated region

VEGF: Vascular endothelial growth factor

WST-1: Water-soluble tetrazolium-1

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