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Abstract
Matrix effect is an important parameter to be investigated during the development and validation of a method for the quantitative determination of contaminants in food. The objective of this study was to evaluate the matrix effect, through statistical tests, in the quantification of amphenicols in fish by HPLC-MS/MS. The study was performed by comparing the standard curves prepared in solvent solutions and in a fish sample previously known to be free of amphenicols. Since matrix effect was observed for the three analytes, calibration curves for quantification of chloramphenicol, thiamphenicol and florfenicol should be constructed using the matrix.
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Details
1 LBqA – Laboratório de Bioquímica de Alimentos – Faculdade de Farmácia, UFMG, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG, BRAZIL
2 Laboratório de Controle de Qualidade de Medicamentos – Faculdade de Farmácia, UFMG, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG, BRAZIL