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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

This study aimed to evaluate the comparative biological effects of Polygonum aviculare L. herba (PAH) extract and quercetin-entrapped liposomes on doxorubicin (Doxo)-induced toxicity in HUVECs. HUVECs were treated with two formulations of liposomes loaded with PAH extract (L5 and L6) and two formulations of liposomes loaded with quercetin (L3 prepared with phosphatidylcholine and L4 prepared with phosphatidylserine). The results obtained with atomic force microscopy, zeta potential and entrapment liposome efficiency confirmed the interactions of the liposomes with PAH or free quercetin and a controlled release of flavonoids entrapped in all the liposomes. Doxo decreased the cell viability and induced oxidative stress, inflammation, DNA lesions and apoptosis in parallel with the activation of Nrf2 and NF-kB. Free quercetin, L3 and L4 inhibited the oxidative stress and inflammation and reduced apoptosis, particularly L3. Additionally, these compounds diminished the Nrf2 and NF-kB expressions and DNA lesions, principally L4. PAH extract, L5 and L6 exerted antioxidant and anti-inflammatory activities, reduced γH2AX formation and inhibited extrinsic apoptosis and transcription factors activation but to a lesser extent. The loading of quercetin in liposomes increased the cell viability and exerted better endothelial protection compared to free quercetin, especially L3. The liposomes with PAH extract had moderate efficiency, mainly due to the antioxidant and anti-inflammatory effects and the inhibition of extrinsic apoptosis.

Details

Title
Comparative Study of the Pharmacological Properties and Biological Effects of Polygonum aviculare L. herba Extract-Entrapped Liposomes versus Quercetin-Entrapped Liposomes on Doxorubicin-Induced Toxicity on HUVECs
Author
Mureşan, Mariana 1 ; Olteanu, Diana 2   VIAFID ORCID Logo  ; Filip, Gabriela Adriana 2   VIAFID ORCID Logo  ; Clichici, Simona 2   VIAFID ORCID Logo  ; Baldea, Ioana 2   VIAFID ORCID Logo  ; Jurca, Tunde 3 ; Pallag, Annamaria 3   VIAFID ORCID Logo  ; Marian, Eleonora 3 ; Frum, Adina 4 ; Gligor, Felicia Gabriela 4   VIAFID ORCID Logo  ; Svera, Paula 5 ; Stancu, Bogdan 6   VIAFID ORCID Logo  ; Vicaș, Laura 3   VIAFID ORCID Logo 

 Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, 10 Piata 1 Decembrie Street, 410073 Oradea, Romania; [email protected] 
 Department of Physiology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 1–3 Clinicilor Street, 400006 Cluj-Napoca, Romania; [email protected] (D.O.); [email protected] (S.C.); [email protected] (I.B.) 
 Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, Romania; [email protected] (T.J.); [email protected] (A.P.); [email protected] (E.M.); [email protected] (L.V.) 
 Faculty of Medicine, Lucian Blaga University Sibiu, Lucian Blaga Street, No. 2A, 550169 Sibiu, Romania; [email protected] (A.F.); [email protected] (F.G.G.) 
 INCEMC-National Institute for Research and Development in Electrochemistry and Condensed Matter-Timisoara, No. 144 Dr. A. Paunescu Podeanu Street, 300569 Timisoara, Romania; [email protected] 
 2nd Department of General Surgery, Iuliu Hatieganu University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania; [email protected] 
First page
1418
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
19994923
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2576478593
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.