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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Growing attention on carcinogenicity and mutagenicity of antineoplastic drugs (ADs) from the International Agencies has led to the present strict safe handling and administration regulations. Accordingly, one of the most common ways to assess occupational exposure to these substances is to identify and quantify possible surface contamination inside hospital preparation and administration units. Thus, it is essential to develop a fast and high-throughput monitoring method capable of identifying a significant number of ADs. The present study reports developing a UHPLC–MS/MS analysis to screen 26 ADs surface contamination through wipe test sampling. A Cortecs UPLC T3 50 × 2.1 mm (1.6 µm) column was selected to perform the analysis, using the evaluations of previous studies and the Product Quality Research Institute (PQRI) database. The design of experiments (DoE) methodological approach was used to optimize the chromatographic conditions concerning the best separation between all ADs. The limits of quantification for the analytes were between the pg/mL and ng/mL orders, and the turnaround time was limited to about 15 min. The obtained accuracy was mostly between 90% and 110% for all the analytes, while the precision was under 10% and a low matrix effect was observed for said analytes. Only vindesine and docetaxel presented lower performances.

Details

Title
Developing a Fast Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method for High-Throughput Surface Contamination Monitoring of 26 Antineoplastic Drugs
Author
Dugheri, Stefano 1   VIAFID ORCID Logo  ; Mucci, Nicola 2   VIAFID ORCID Logo  ; Squillaci, Donato 2   VIAFID ORCID Logo  ; Marrubini, Giorgio 3   VIAFID ORCID Logo  ; Bartolucci, Gianluca 4   VIAFID ORCID Logo  ; Melzi, Camillo 5 ; Bucaletti, Elisabetta 2 ; Cappelli, Giovanni 2 ; Trevisani, Lucia 2 ; Arcangeli, Giulio 2 

 Industrial Hygiene and Toxicology Laboratory, Occupational Medicine Unit, Careggi University Hospital, 50134 Florence, Italy 
 Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy; [email protected] (N.M.); [email protected] (D.S.); [email protected] (E.B.); [email protected] (G.C.); [email protected] (L.T.); [email protected] (G.A.) 
 Department of Drug Sciences, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy; [email protected] 
 Department of Neurosciences, Psychology, Drug Research and Child Health, University of Florence, 50019 Sesto Fiorentino, Italy; [email protected] 
 Metanalisi, Corso di Porta Venezia 40, 20121 Milano, Italy; [email protected] 
First page
150
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
22978739
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2576485192
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.