Abstract

The purpose of this study was to elucidate, under which conditions abattoir-acquired pig eyes are suitable for refractive excimer laser experiments. Porcine eyes from tunnel-scalded (n = 5) and tank-scalded (n = 10) pigs were compared to unscalded eyes (n = 5) and to eyes scalded in the laboratory (n = 5). The corneal epithelium was removed before an excimer laser was used to perform a − 8.0 D photoablation. Corneal thickness was measured by optical coherence topography before and after photoablation. The ablation depth was determined with a contour measuring station, the morphology of the ablated areas was characterized by scanning electron microscopy and white-light profilometry. The scalded eyes showed an increase in corneal swelling which gained statistical significance in tank-scalded eyes showing a wedge-shaped opaque stromal lesion in the nasal corneal quadrant. A measurable deterioration of photoablation was only found in tank-scalded eyes that exhibited the opaque lesion. Ablated area morphology was smooth and regular in the unscalded and tunnel-scalded eyes. The tank-scalded eyes showed conspicuous wrinkles. While unscalded eyes should always be preferred for excimer laser laboratory experiments, the data suggest that the use of tunnel-scalded eyes may also be acceptable and should be chosen over tank-scalded eyes.

Details

Title
Usability of abattoir-acquired pig eyes for refractive excimer laser research
Author
Topka Marius 1 ; Zhang, Yao 2 ; Bock, Antonia 2 ; Riedel, Peter 2 ; Lörner Johannes 2 ; Hammer, Alexander 3 ; Maier, Eva 4 ; Paulsen Friedrich 5 ; Hammer, Christian M 1 

 Friedrich-Alexander University Erlangen-Nürnberg, Institute of Functional and Clinical Anatomy, Erlangen, Germany (GRID:grid.5330.5) (ISNI:0000 0001 2107 3311) 
 WaveLight GmbH, Erlangen, Germany (GRID:grid.467675.1) (ISNI:0000 0004 0629 4302) 
 Paracelsus Medical University, Department of Neurosurgery, Nürnberg, Germany (GRID:grid.467675.1) 
 Friedrich-Alexander University Erlangen-Nürnberg, Dental Clinic 1, Operative Dentistry and Periodontology, Erlangen, Germany (GRID:grid.5330.5) (ISNI:0000 0001 2107 3311) 
 Friedrich-Alexander University Erlangen-Nürnberg, Institute of Functional and Clinical Anatomy, Erlangen, Germany (GRID:grid.5330.5) (ISNI:0000 0001 2107 3311); Sechenov University, Department of Operative Surgery and Topographic Anatomy, Moscow, Russia (GRID:grid.448878.f) (ISNI:0000 0001 2288 8774) 
Publication year
2021
Publication date
2021
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2576737739
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.