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Abstract
The endocytic compartments keep their interior acidic through the inward flow of protons and anions from the cytosol. Acidification is mediated by a proton pump known as vacuolar-type ATPase (V-ATPase) and transporters conferring anion conductance to the organellar membrane. In this study, we analysed the phenotype of mouse embryos lacking the V-ATPase c-subunit. The mutant embryos differentiated embryonic epithelial tissues, primitive endoderm, epiblast, and extraembryonic ectoderm; however, the organisation of these epithelia was severely affected. The apical-basal polarity in the visceral endoderm layer was not properly established in the mutant embryos, resulting in abnormal epithelial morphology. Thus, the function of V-ATPase is imperative for the establishment and/or maintenance of epithelial cell polarity, which is required for early embryogenesis.
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Details
1 Doshisha Women’s College of Liberal Arts, Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kohdo, Kyotanabe, Japan (GRID:grid.444204.2) (ISNI:0000 0001 0193 2713)
2 Doshisha Women’s College of Liberal Arts, Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kohdo, Kyotanabe, Japan (GRID:grid.444204.2) (ISNI:0000 0001 0193 2713); Himeji Dokkyo University, Division of Biochemistry, Faculty of Pharmaceutical Sciences, Himeji, Japan (GRID:grid.412142.0) (ISNI:0000 0000 8894 6108)
3 Institute of Scientific and Industrial Research, Osaka University, Division of Biological Sciences, Ibaraki, Japan (GRID:grid.136593.b) (ISNI:0000 0004 0373 3971)