Abstract

Tonsil-derived mesenchymal stem cells (TMSCs) showed therapeutic effects on acute and chronic murine colitis models, owing to their immunomodulatory properties; therefore, we evaluated enhanced therapeutic effects of TMSCs on a murine colitis model using three-dimensional (3D) culture method. The expression of angiogenic factors, VEGF, and anti-inflammatory cytokines, IL-10, TSG-6, TGF-β, and IDO-1, was significantly higher in the 3D-TMSC-treated group than in the 2D-TMSC-treated group (P < 0.05). At days 18 and 30 after inducing chronic colitis, disease activity index scores were estimated to be significantly lower in the 3D-TMSC-treated group than in the colitis control (P < 0.001 and P < 0.001, respectively) and 2D-TMSC-treated groups (P = 0.022 and P = 0.004, respectively). Body weight loss was significantly lower in the 3D-TMSC-treated group than in the colitis control (P < 0.001) and 2D-TMSC-treated groups (P = 0.005). Colon length shortening was significantly recovered in the 3D-TMSC-treated group compared to that in the 2D-TMSC-treated group (P = 0.001). Histological scoring index was significantly lower in the 3D-TMSC-treated group than in the 2D-TMSC-treated group (P = 0.002). These results indicate that 3D-cultured TMSCs showed considerably higher therapeutic effects in a chronic murine colitis model than those of 2D-cultured TMSCs via increased anti-inflammatory cytokine expression.

Details

Title
Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
Author
Song, Eun Mi 1 ; Joo, Yang Hee 1 ; Reum, Choe A 1 ; Park Yehyun 1 ; Tae Chung Hyun 1 ; Hong Ji Teak 1 ; Moon Chang Mo 1 ; Seong-Eun, Kim 1 ; Jung, Hye-Kyung 1 ; Ki-Nam, Shim 1 ; Cho Kyung-Ah 2 ; Inho, Jo 3 ; Sung-Ae, Jung 1 

 Ewha Womans University, Department of Internal Medicine, College of Medicine, Seoul, South Korea (GRID:grid.255649.9) (ISNI:0000 0001 2171 7754) 
 Ewha Womans University, Department of Microbiology, College of Medicine, Seoul, South Korea (GRID:grid.255649.9) (ISNI:0000 0001 2171 7754) 
 Ewha Womans University, Department of Molecular Medicine, College of Medicine, Seoul, South Korea (GRID:grid.255649.9) (ISNI:0000 0001 2171 7754) 
Publication year
2021
Publication date
2021
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2578267882
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.