A novel fluorescent probe for detecting hydrogen sulfide in osteoblasts during lipopolysaccharide-mediated inflammation under periodontitis

Abstract

Periodontitis, one of the most common chronic inflammatory diseases, affects the quality of life. Osteogenesis plays an important role in the disease. There is a connection between hydrogen sulfide (H₂S) and periodontitis, but according to the study has been published, the precise role of H₂S in inflammation remains in doubt. The main reason for the lack of research is that H₂S is an endogenous gasotransmitter, difficult to discern through testing. So, we synthesized a novel fluorescence probe which can detect H₂S in vitro. By using the novel H₂S fluorescence probe, we found that H₂S changes in osteoblasts mainly by cystathionine-γ-lyase, and H₂S increases under LPS stimulation. H₂S could be a potential marker for diagnosis of inflammatory diseases of bone, and might help deepen studies of the changes of H₂S level and promote the progression on the researches about pathogenesis of periodontitis.