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Abstract
Sulphate-reducing bacteria (SRB) cause fouling, souring, corrosion and produce H2S during oil and gas production. Produced water obtained from Periquito (PQO) and Galo de Campina (GC) onshore oilfields in Brazil was investigated for SRB. Produced water with Postgate B, Postgate C and Baars media was incubated anaerobically for 20 days. DNA was extracted, 16S rDNA PCR amplified and fragments were sequenced using Illumina TruSeq. 4.2 million sequence reads were analysed and deposited at NCBI SAR accession number SRP149784. No significant differences in microbial community composition could be attributed to the different media but significant differences in the SRB were observed between the two oil fields. The dominant bacterial orders detected from both oilfields were Desulfovibrionales, Pseudomonadales and Enterobacteriales. The genus Pseudomonas was found predominantly in the GC oilfield and Pleomorphominas and Shewanella were features of the PQO oilfield. 11% and 7.6% of the sequences at GC and PQO were not classified at the genus level but could be partially identified at the order level. Relative abundances changed for Desulfovibrio from 29.8% at PQO to 16.1% at GC. Clostridium varied from 2.8% at PQO and 2.4% at GC. These data provide the first description of SRB from onshore produced water in Brazil and reinforce the importance of Desulfovibrionales, Pseudomonadales, and Enterobacteriales in produced water globally. Identifying potentially harmful microbes is an important first step in developing microbial solutions that prevent their proliferation.
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1 Universidade Federal do Rio de Janeiro (UFRJ), Post Graduate Program in Plant Biotechnology and Bioprocesses, Decania, Center for Health Sciences, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X)
2 Universidade Federal do Rio de Janeiro (UFRJ), Post Graduate Program in Plant Biotechnology and Bioprocesses, Decania, Center for Health Sciences, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X); Universidade Federal do Rio de Janeiro (UFRJ), Institute of Microbiology Paulo de Góes, Brasil, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X)
3 Universidade Federal do Rio de Janeiro (UFRJ), Post Graduate Program in Plant Biotechnology and Bioprocesses, Decania, Center for Health Sciences, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X); Universidade Federal do Rio de Janeiro (UFRJ), Institute of Microbiology Paulo de Góes, Brasil, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X); King Abdullah University of Science and Technology (KAUST), Red Sea Research Center (RSRC), Division of Biological and Environmental Science and Engineering (BESE), Thuwal, Saudi Arabia (GRID:grid.45672.32) (ISNI:0000 0001 1926 5090)
4 Universidade Federal do Rio de Janeiro (UFRJ), Institute of Microbiology Paulo de Góes, Brasil, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X)
5 Universidade Federal do Rio de Janeiro (UFRJ), Institute of Microbiology Paulo de Góes, Brasil, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X); Universidade Federal do Rio de Janeiro (UFRJ), BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy Lab, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X)
6 Universidade Federal do Rio de Janeiro (UFRJ), Post Graduate Program in Plant Biotechnology and Bioprocesses, Decania, Center for Health Sciences, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X); North Carolina State University, Department of Molecular and Structural Biochemistry, Raleigh, USA (GRID:grid.40803.3f) (ISNI:0000 0001 2173 6074)
7 Capital Intelectual Instituto Interdisciplinar de P&D (C3i), Limeira, Brazil (GRID:grid.40803.3f)
8 Universidade Federal do Rio de Janeiro (UFRJ), Post Graduate Program in Plant Biotechnology and Bioprocesses, Decania, Center for Health Sciences, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X); Universidade Federal do Rio de Janeiro (UFRJ), Institute of Microbiology Paulo de Góes, Brasil, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X); Universidade Federal do Rio de Janeiro (UFRJ), BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy Lab, Rio de Janeiro, Brazil (GRID:grid.8536.8) (ISNI:0000 0001 2294 473X)