Abstract

Cardiovascular disease (CVD) and type 2 diabetes (T2D) are increasing worldwide. This is mainly due to an unhealthy nutrition, implying that variation in CVD risk may be due to variation in the capacity to manage a nutritional load. We examined the genomic basis of postprandial metabolism. Our main purpose was to introduce the GEMM Family Study (Genetics of Metabolic Diseases in Mexico) as a multi-center study carrying out an ongoing recruitment of healthy urban adults. Each participant received a mixed meal challenge and provided a 5-hours’ time course series of blood, buffy coat specimens for DNA isolation, and adipose tissue (ADT)/skeletal muscle (SKM) biopsies at fasting and 3 h after the meal. A comprehensive profiling, including metabolomic signatures in blood and transcriptomic and proteomic profiling in SKM and ADT, was performed to describe tendencies for variation in postprandial response. Our data generation methods showed preliminary trends indicating that by characterizing the dynamic properties of biomarkers with metabolic activity and analyzing multi-OMICS data it could be possible, with this methodology and research design, to identify early trends for molecular biology systems and genes involved in the fasted and fed states.

Details

Title
Deep Multi-OMICs and Multi-Tissue Characterization in a Pre- and Postprandial State in Human Volunteers: The GEMM Family Study Research Design
Author
Bastarrachea, Raul A 1 ; Laviada-Molina, Hugo A 2 ; Nava-Gonzalez, Edna J 3 ; Leal-Berumen, Irene 4   VIAFID ORCID Logo  ; Escudero-Lourdes, Claudia 5   VIAFID ORCID Logo  ; Escalante-Araiza, Fabiola 6 ; Peschard, Vanessa-Giselle 6 ; Veloz-Garza, Rosa A 7 ; Haack, Karin 1 ; Martínez-Hernández, Angélica 8 ; Barajas-Olmos, Francisco M 8 ; Molina-Segui, Fernanda 2 ; Buenfil-Rello, Fatima A 2 ; Gonzalez-Ramirez, Lucia 2 ; Janssen-Aguilar, Reinhard 2 ; Lopez-Muñoz, Ricardo 2 ; Perez-Cetina, Fernanda 1 ; Gaytan-Saucedo, Janeth F 1 ; Vaquera, Zoila 1 ; Cornejo-Barrera, Judith 9 ; Castillo-Pineda, Juan Carlos 10 ; Murillo-Ramirez, Areli 10 ; Diaz-Tena, Sara P 10 ; Figueroa-Nuñez, Benigno 11 ; González-López, Laura 12 ; Salinas-Osornio, Rocío A 12 ; Valencia-Rendón, Melesio E 12 ; Ángeles-Chimal, José 13   VIAFID ORCID Logo  ; Santa-Olalla Tapia, Jesús 13   VIAFID ORCID Logo  ; Remes-Troche, José M 14   VIAFID ORCID Logo  ; Valdovinos-Chavez, Salvador B 7 ; Huerta-Avila, Eira E 1 ; Han, Xianlin 15   VIAFID ORCID Logo  ; Orozco, Lorena 8 ; Rodriguez-Ayala, Ernesto 6 ; Weintraub, Susan 16 ; Gallegos-Cabrales, Esther C 7 ; Cole, Shelley A 1 ; KentJr, Jack W 1 

 Department of Genetics, Texas Biomedical Research Institute, San Antonio, TX 78227-0549, USA 
 Escuela de Ciencias de la Salud, Universidad Marista de Mérida, Mérida 97300, Mexico 
 Facultad de Salud Pública y Nutrición (FASPyN), UANL, Monterrey 64460, Mexico 
 Facultad de Medicina y Ciencias Biomédicas, Universidad Autónoma de Chihuahua, Chihuahua 31125, Mexico 
 Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí 78240, Mexico 
 Centro de Investigación en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud, Universidad Anáhuac Norte, Lomas Anahuac 52786, Mexico 
 Facultad de Enfermería, Universidad Autónoma de Nuevo León (UANL), Monterrey 64460, Mexico 
 Laboratorio de Inmunogenómica y Enfermedades Metabólicas, Instituto Nacional de Medicina Genómica, Ciudad de México C.P. 14610, Mexico 
 Departamento de Enseñanza, Postgrado e Investigación, Hospital Infantil de Tamaulipas, Ciudad Victoria 87150, Mexico 
10  Departamento de Nutrición Humana, Universidad Latina de América, Morelia, Michoacán 58170, Mexico 
11  Clínica de Enfermedades Crónicas y Procedimientos Especiales (CECYPE), Morelia 58249, Mexico 
12  Universidad del Valle de Atemajac (UNIVA), Zapopan, Jalisco 45050, Mexico 
13  Facultad de Medicina, Universidad Autónoma Estado de Morelos, Cuernavaca 62209, Mexico 
14  Instituto de Investigaciones Médico-Biológicas, Universidad Veracruzana, Veracruz 91700, Mexico 
15  Department of Medicine, Sam and Ann Barshop Institute for Longevity and Aging Studies, University of Texas Health San Antonio, San Antonio, TX 78229, USA 
16  Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229, USA 
First page
532
Publication year
2018
Publication date
2018
Publisher
MDPI AG
e-ISSN
20734425
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2582804761
Copyright
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.