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© 2020. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

BARD1 is associated with the development of high-risk neuroblastoma patients. Particularly, the expression of full length (FL) isoform, FL BARD1, correlates to high-risk neuroblastoma development and its inhibition is sufficient to induce neuroblastoma cells towards a worst phenotype. Here we have investigated the mechanisms of FL BARD1 in neuroblastoma cell lines depleted for FL BARD1 expression. We have shown that FL BARD1 expression protects the cells from spontaneous DNA damage and from damage accumulated after irradiation. We demonstrated a role for FL BARD1 as tumor suppressor to prevent unscheduled mitotic entry of DNA damaged cells and to lead to death cells that have bypassed cell cycle checkpoints. FL BARD1-depleted cells that have survived to checkpoints acquire features of aggressiveness. Overall, our results show that FL BARD1 may defend cells against cancer and prevent malignant transformation of cells.

Details

Title
Functional characterization of full-length BARD1 strengthens its role as a tumor suppressor in neuroblastoma
Author
Cimmino, Flora; Avitabile, Marianna; Lasorsa, Vito Alessandro; Pezone, Lucia; Cardinale, Antonella; Montella, Annalaura; Cantalupo, Sueva; Iolascon, Achille; Capasso, Mario
Pages
1495-1504
Section
Research Papers
Publication year
2020
Publication date
2020
Publisher
Ivyspring International Publisher Pty Ltd
e-ISSN
18379664
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2598355276
Copyright
© 2020. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.