Full text

Turn on search term navigation

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Rhabdoviridae is the most diverse family of the negative, single-stranded RNA viruses, which includes 40 ecologically different genera that infect plants, insects, reptiles, fishes, and mammals, including humans, and birds. To date, only a few bird-related rhabdoviruses among the genera Sunrhavirus, Hapavirus, and Tupavirus have been described and analyzed at the molecular level. In this study, we characterized seven additional and previously unclassified rhabdoviruses, which were isolated from various bird species collected in Africa during the 1960s and 1970s. Based on the analysis of their genome sequences obtained by next generation sequencing, we observed a classical genomic structure, with the presence of the five canonical rhabdovirus genes, i.e., nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and polymerase (L). In addition, different additional open reading frames which code putative proteins of unknown function were identified, with the common presence of the C and the SH proteins, within the P gene and between the M and G genes, respectively. Genetic comparisons and phylogenetic analysis demonstrated that these seven bird-related rhabdoviruses could be considered as putative new species within the genus Sunrhavirus, where they clustered into a single group (named Clade III), a companion to two other groups that encompass mainly insect-related viruses. The results of this study shed light on the high diversity of the rhabdoviruses circulating in birds, mainly in Africa. Their close relationship with other insect-related sunrhaviruses raise questions about their potential role and impact as arboviruses that affect bird communities.

Details

Title
Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa
Author
Dong-Sheng, Luo 1 ; Zhi-Jian Zhou 2 ; Xing-Yi, Ge 2   VIAFID ORCID Logo  ; Bourhy, Hervé 3 ; Zheng-Li, Shi 4   VIAFID ORCID Logo  ; Grandadam, Marc 5 ; Dacheux, Laurent 3   VIAFID ORCID Logo 

 CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; [email protected] (D.-S.L.); [email protected] (Z.-L.S.); University of Chinese Academy of Sciences, Beijing 100049, China; Lyssavirus Epidemiology and Neuropathology Unit, Institut Pasteur, Université de Paris, 75015 Paris, France; [email protected] 
 Hunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China; [email protected] (Z.-J.Z.); [email protected] (X.-Y.G.) 
 Lyssavirus Epidemiology and Neuropathology Unit, Institut Pasteur, Université de Paris, 75015 Paris, France; [email protected] 
 CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; [email protected] (D.-S.L.); [email protected] (Z.-L.S.); University of Chinese Academy of Sciences, Beijing 100049, China 
 Institut de Recherche Biomédicale des Armées, 91220 Bretigny-sur-Orge, France; [email protected]; National Reference Center for Arboviruses, Institut Pasteur, Université de Paris, 75015 Paris, France 
First page
2168
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
19994915
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2602201403
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.