Abstract

Background

The purpose of the present study was to evaluate the efficacy of chemo-magnetic hyperthermia (MH), a combination of alternating magnetic field (AMF) and superparamagnetic nanoparticles (SPIONs) coated with Polyethylene glycol-Poly(butyl acrylate)-Polyethylene glycol (PEG-PBA-PEG) carrying 5-Fluorouracil (5-Fu), at inducing apoptosis in the human cancer cell lines HT29 and HCT116. This process can be mediated by alterations in the expression of apoptotic effector proteins, including Bax, Bcl-2, cleaved caspase 3&9, and cleaved PARP, which are involved in the intrinsic pathway of apoptosis. For this purpose, the cells were cultured as monolayers. Then both cell lines were treated with 5-Fu/magnetic nanoparticles and magnetic hyperthermia. Finally, the effect of treatment on cancer cells was determined by Western blot analysis and flow cytometry.

Results

Our results showed that combined chemo-magnetic thermotherapy significantly increased the apoptosis in colon cancer cells compared to chemotherapy or hyperthermia alone (P < 0.05). Up-regulation of Bax, cleaved caspase 3&9, and cleaved PARP proteins was indicative of apoptosis induction in cancer cells, which are involved in the intrinsic pathway of apoptosis.

Conclusions

This study demonstrates that localized hyperthermia was able to significantly trigger the 5-Fu release and inhibit cell viability, which, due to the synchronization of hyperthermia and chemotherapy, exacerbated the damage of cancer cells.

Details

Title
Potential anti-tumor activity of 13.56 MHz alternating magnetic hyperthermia and chemotherapy on the induction of apoptosis in human colon cancer cell lines HT29 and HCT116 by up-regulation of Bax, cleaved caspase 3&9, and cleaved PARP proteins
Author
Jahangiri Saba 1 ; Khoei Samideh 2   VIAFID ORCID Logo  ; Khoee Sepideh 3 ; Safa Majid 4 ; Shirvalilou Sakine 5 ; Vahid, Pirhajati Mahabadi 6 

1  Iran University of Medical Sciences, Student Research Committee, Tehran, Iran (GRID:grid.411746.1) (ISNI:0000 0004 4911 7066); Iran University of Medical Sciences, Department of Medical Physics, School of Medicine, Tehran, Iran (GRID:grid.411746.1) (ISNI:0000 0004 4911 7066) 
2  Iran University of Medical Sciences, Finetech in Medicine Research Center, Tehran, Iran (GRID:grid.411746.1) (ISNI:0000 0004 4911 7066); Iran University of Medical Sciences, Department of Medical Physics, School of Medicine, Tehran, Iran (GRID:grid.411746.1) (ISNI:0000 0004 4911 7066) 
3  University of Tehran, Department of Polymer Chemistry, School of Chemistry, College of Science, Tehran, Iran (GRID:grid.46072.37) (ISNI:0000 0004 0612 7950) 
4  Iran University of Medical Sciences, Department of Hematology and Blood Transfusion, Faculty of Allied Medicine, Tehran, Iran (GRID:grid.411746.1) (ISNI:0000 0004 4911 7066) 
5  Iran University of Medical Sciences, Finetech in Medicine Research Center, Tehran, Iran (GRID:grid.411746.1) (ISNI:0000 0004 4911 7066) 
6  Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran (GRID:grid.411746.1) (ISNI:0000 0004 4911 7066) 
Publication year
2021
Publication date
Dec 2021
Publisher
Springer Nature B.V.
ISSN
18686958
e-ISSN
18686966
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s12645-021-00108-5
ProQuest document ID
2610661437
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.