Abstract

This study aimed to synthesize Melphalan (MEL)-loaded PEGylated nanoliposomes and evaluate their cytotoxic effects on ovarian cancer A2780CP and SKOV3 cells. For this purpose, MEL-loaded PEGylated nanoliposomes were prepared by the reverse-phase evaporation method. They were then characterized, and the results showed that the size, size distribution, and zeta potential of the nanoformulation were 129.1 ± 5.5 nm, 0.240 ± 0.13, and −18.9  0.31 mv, respectively. Also, the encapsulation and loading efficiencies of MEL in the nanoparticles were determined to be 98.3 ± 2.1% and 5.2 ± 1.3%, respectively. Also, the drug release pattern was evaluated using a dialysis bag method, and the results demonstrated that 20.5% of MEL was released after 48 h from the nanoparticles, which was 2.7-fold slower compared to when the standard drug was used. In addition, the effects of the standard drug and nanoformulation on cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the results showed that nanoformulation had 1.4-fold more toxicity than the standard drug. Moreover, the extent of apoptosis was investigated using the Annexin- propidium iodide kit and flow cytometry method. The results showed that MEL-loaded PEGylated nanoliposomes increased the apoptotic cells compared to the control group.

Details

Title
Investigation of characterization and cytotoxic effect of PEGylated nanoliposomal containing Melphalan on ovarian cancer: an in vitro study
Author
Seyedeh Masoomeh Sadat Mirnezami 1 ; Heydarinasab, Amir 1 ; Akbarzadeh, Azim 2 ; Arjmand, Mehdi 3 

 Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran 
 Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran 
 Department of Chemical Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran 
Pages
101-115
Publication year
2021
Publication date
Dec 2021
Publisher
Taylor & Francis Ltd.
ISSN
17458080
e-ISSN
17458099
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2611009317
Copyright
© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.