It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
Usutu virus (USUV) is a zoonotic arbovirus causing avian mass mortalities. The first outbreak in North-Western Germany occurred in 2018. This retrospective analysis focused on combining virological and pathological findings in birds and immunohistochemistry. 25 common blackbirds, one great grey owl, and one kingfisher collected from 2011 to 2018 and positive for USUV by qRT-PCR were investigated. Macroscopically, most USUV infected birds showed splenomegaly and hepatomegaly. Histopathological lesions included necrosis and lymphohistiocytic inflammation within spleen, Bursa fabricii, liver, heart, brain, lung and intestine. Immunohistochemistry revealed USUV antigen positive cells in heart, spleen, pancreas, lung, brain, proventriculus/gizzard, Bursa fabricii, kidney, intestine, skeletal muscle, and liver. Analysis of viral genome allocated the virus to Europe 3 or Africa 2 lineage. This study investigated whether immunohistochemical detection of double-stranded ribonucleic acid (dsRNA) serves as an alternative tool to detect viral intermediates. Tissue samples of six animals with confirmed USUV infection by qRT-PCR but lacking viral antigen in liver and spleen, were further examined immunohistochemically. Two animals exhibited a positive signal for dsRNA. This could indicate either an early state of infection without sufficient formation of virus translation products, occurrence of another concurrent virus infection or endogenous dsRNA not related to infectious pathogens and should be investigated in more detail in future studies.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 University of Veterinary Medicine Hanover Foundation, Department of Pathology, Hannover, Germany (GRID:grid.412970.9) (ISNI:0000 0001 0126 6191)
2 University of Veterinary Medicine Hanover, Research Center for Emerging Infections and Zoonoses, Hanover, Germany (GRID:grid.412970.9) (ISNI:0000 0001 0126 6191)
3 Chemisches Und Veterinäruntersuchungsamt Westfalen, Arnsberg, Germany (GRID:grid.412970.9)
4 Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Greifswald-Insel Riems, Germany (GRID:grid.417834.d)
5 University of Veterinary Medicine Hanover, Research Center for Emerging Infections and Zoonoses, Hanover, Germany (GRID:grid.412970.9) (ISNI:0000 0001 0126 6191); University of Veterinary Medicine Hanover, Institute for Biochemistry, Hanover, Germany (GRID:grid.412970.9) (ISNI:0000 0001 0126 6191)
6 University of Veterinary Medicine Hanover Foundation, Department of Pathology, Hannover, Germany (GRID:grid.412970.9) (ISNI:0000 0001 0126 6191); Leipzig University, Institute of Veterinary-Pathology, Leipzig, Germany (GRID:grid.9647.c) (ISNI:0000 0004 7669 9786)