Abstract

Characterization of a green algae Lobochlamys segnis strain 019 using morphological dan phylogenetic study were determined. In this study, contribution of natural nutrients will be evaluated by culturing this strain using Sphagnum peat soil extract in comparing to that of a commercial media for freshwater algae. Based on morphological study, L.segnis strain 019 is a unicellular biflagellate. This Chlamydomonas-like algae possessed a cup shaped to lateral chloroplast with central pyrenoid and a low indistinct papilla. Molecular phylogenetic analyses based on 18 S rDNA indicated that this strain is a member of Lobochlamys subclade, and formed a robust clade (PP = 1.0, BP = 99%) with Oogamochlamys. Strain 019 formed a buble-like colonies covered by mucilage material under agar culture condition. In this study, a moderate acidic condition pH 4.0 was applied for both media due to liquid medium of Sphagnum peat soil extract detected in this pH value. The biomass production, lipid production and fatty acid composition using peat soil extract and AF-6 media are evaluated and discussed.

Details

Title
Study of a green algae Lobochlamys segnis Strain-019 from peatland
Author
Susanti, H 1 ; Nakayama, T 2 

 Research Center for Biotechnology, Research Organization for Life Science, National Research and Innovation Agency (BRIN), Cibinong 16911, West Java, Indonesia 
 Faculty of Life and Environmental Sciences, University of Tsukuba 1-1-1 Tennodai Tsukuba 305-8577 Japan 
Publication year
2021
Publication date
Dec 2021
Publisher
IOP Publishing
ISSN
17551307
e-ISSN
17551315
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2612140008
Copyright
© 2021. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.