Abstract

All parts of Thanakha (Hesperethusa crenulata R.) have been used as traditional skin care herbal material in Myanmar. In this study, coumarins from H. crenulata R. bark were isolated through solvent extraction, systematic solvent fractionation, and repeated column chromatography. Spectroscopic analyses using ESI–MS, 1D NMR (1H and 13C), 2D NMR (gHSQC and gHMBC), specific rotation, circular dichroism, and IR spectrometry revealed three coumarins 2R-7-hydroxy-8-(2,3-dihydroxy-3-methylbutyl)-coumarin (compound 1), peucedanol (compound 2), and methylpeucedanol (compound 3), which were first isolated from Thanakha tree. Antioxidant capacities of three coumarins decreased as follows: compound 2 > compound 3 > compound 1. Treatments of lipopolysaccharide-induced THP-1 human monocytic cells with compounds 2 and 3 at 378.8 μM and 359.7 μM inhibited tumor necrosis factor-α production by approximately 32.7% and 13.3%, respectively, compared with the negative control. In summary, these results suggest that Thanakha bark extracts can be used as a potent antioxidant and anti-inflammatory source for cosmetic ingredients.

Details

Title
Antioxidant and anti-inflammatory effects in lipopolysaccharide-induced THP-1 cells of coumarins from the bark of Hesperethusa crenulata R.
Author
Seung-Su, Lee 1 ; Kim Hyoung-Geun 1 ; Eun-Ha, Park 2 ; Kim, Kwang Joong 2 ; Myun-Ho, Bang 3 ; Kim, Gayoung 1 ; Jeon Hyeong-Ju 4 ; Chung-Gi, Lee 5 ; Min-Chul, Shin 5 ; Dae-Ok, Kim 6 ; Baek Nam-In 1 

 Kyung Hee University, Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Yongin, Republic of Korea (GRID:grid.289247.2) (ISNI:0000 0001 2171 7818) 
 Kyung Hee University, Department of Food Science and Biotechnology, Yongin, Republic of Korea (GRID:grid.289247.2) (ISNI:0000 0001 2171 7818) 
 Kyung Hee University, Skin Biotechnology Center, Suwon, Republic of Korea (GRID:grid.289247.2) (ISNI:0000 0001 2171 7818) 
 Jangan University, Department of Food and Nutrition, Hwaseong, Republic of Korea (GRID:grid.495942.7) (ISNI:0000 0004 6018 4876) 
 Cytus H&B, Cheongju, Republic of Korea (GRID:grid.495942.7) 
 Kyung Hee University, Department of Food Science and Biotechnology, Yongin, Republic of Korea (GRID:grid.289247.2) (ISNI:0000 0001 2171 7818); Kyung Hee University, Skin Biotechnology Center, Suwon, Republic of Korea (GRID:grid.289247.2) (ISNI:0000 0001 2171 7818) 
Publication year
2021
Publication date
Dec 2021
Publisher
Springer Nature B.V.
ISSN
24680834
e-ISSN
24680842
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2612562986
Copyright
© The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.