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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

A novel greener MNC/PES membrane was developed through an electrospinning technique for lipase immobilization to catalyze the synthesis of ethyl valerate (EV). In this study, the covalent immobilization of Aspergillus oryzae lipase (AOL) onto an electrospun nanofibrous membrane consisting of magnetic nanocellulose (MNC) and polyethersulfone (PES) to produce EV was statistically optimized. Raman spectroscopy, Fourier-transform infrared spectroscopy: attenuated total reflection, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, thermal gravimetric analysis (TGA), and differential thermal gravimetric (DTG) of MNC/PES-AOL demonstrated that AOL was successfully immobilized onto the fibers. The Taguchi design-assisted immobilization of AOL onto MNC/PES fibers identified that 1.10 mg/mL protein loading, 4 mL reaction volume, 250 rpm stirring rate, and 50 °C were optimal to yield 72.09% of EV in 24 h. The thermal stability of MNC/PES-AOL was improved by ≈20% over the free AOL, with reusability for up to five consecutive esterification cycles while demonstrating an exceptional half-life of 120 h. Briefly, the electrospun MNC/PES fibers that immobilized AOL showed promising applicability in yielding relatively good EV levels. This study suggests that using MNC as fillers in a PES to improve AOL activity and durability for a longer catalytic process could be a viable option.

Details

Title
Electrospun Magnetic Nanocellulose–Polyethersulfone-Conjugated Aspergillus oryzae Lipase for Synthesis of Ethyl Valerate
Author
Hussin, Nurul Hidayah 1 ; Roswanira Abdul Wahab 2   VIAFID ORCID Logo  ; Nursyafiqah Elias 1   VIAFID ORCID Logo  ; Jacob, Adikwu Gowon 3 ; Mohamad Hamdi Zainal-Abidin 4   VIAFID ORCID Logo  ; Faizuan Abdullah 4 ; Sulaiman, Nurul Jannah 5 ; Misson, Mailin 6   VIAFID ORCID Logo 

 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia; [email protected] (N.H.H.); [email protected] (N.E.); [email protected] (A.G.J.); [email protected] (M.H.Z.-A.); [email protected] (F.A.); Enzyme Technology and Green Synthesis Group, Faculty of Science, Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia 
 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia; [email protected] (N.H.H.); [email protected] (N.E.); [email protected] (A.G.J.); [email protected] (M.H.Z.-A.); [email protected] (F.A.); Enzyme Technology and Green Synthesis Group, Faculty of Science, Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia; Advanced Membrane Technology Research Centre (AMTEC), Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia 
 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia; [email protected] (N.H.H.); [email protected] (N.E.); [email protected] (A.G.J.); [email protected] (M.H.Z.-A.); [email protected] (F.A.); Enzyme Technology and Green Synthesis Group, Faculty of Science, Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia; Department of Applied Chemistry, Federal University Dutsin-Ma (FUDMA), Dutsin-Ma P.M.B 5001, Katsina State, Nigeria 
 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia; [email protected] (N.H.H.); [email protected] (N.E.); [email protected] (A.G.J.); [email protected] (M.H.Z.-A.); [email protected] (F.A.) 
 Department of Bioprocess & Polymer Engineering, School of Chemical & Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia; [email protected] 
 Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia 
First page
972
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
20770375
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2612814415
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.