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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Spinal Cord Injury (SCI) promotes a cascade of inflammatory events that are responsible for neuronal death and glial scar formation at the site of the injury, hindering tissue neuroregeneration. Among the main approaches for the treatment of SCI, the use of biomaterials, especially gelatin methacryloyl (GelMA), has been proposed because it is biocompatible, has excellent mechanical properties, favoring cell adhesion and proliferation. In addition, it can act as a carrier of anti-inflammatory drugs, preventing the formation of glial scars. The present work presents the development and in situ application of a light-curing formulation based on GelMA containing a natural extract rich in anti-inflammatory, antioxidant and neuroprotective substances (hydroalcoholic extract of red propolis—HERP) in an experimental model of SCI in rats. The formulations were prepared and characterized by time of UV exposition, FTIR, swelling and degradation. The hydrogels containing 1 mg/mL of HERP were obtained by the exposure to UV radiation of 2 μL of the formulation for 60 s. The locomotor evaluation of the animals was performed by the scale (BBB) and demonstrated that after 3 and 7 days of the injury, the GelMA-HERP group (BBB = 5 and 7) presented greater recovery compared to the GelMA group (BBB = 4 and 5). Regarding the inflammatory process, using histomorphological techniques, there was an inflammation reduction in the groups treated with GelMA and GelMA-HERP, with decreases of cavitation in the injury site. Therefore, it is possible to conclude that the use of GelMA and GelMA-HERP hydrogel formulations is a promising strategy for the treatment of SCI when applied in situ, as soon as possible after the injury, improving the clinical and inflammatory conditions of the treated animals.

Details

Title
Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury
Author
Novais, Gabrielle B 1 ; Stefane dos Santos 2 ; Santana, Robertta J R 3 ; Filho, Rose N P 4 ; Cunha, John L S 5 ; Lima, Bruno S 6 ; Araújo, Adriano A S 6 ; Severino, Patricia 7   VIAFID ORCID Logo  ; Ricardo L C Albuquerque Júnior 4 ; Cardoso, Juliana C 8   VIAFID ORCID Logo  ; Souto, Eliana B 9   VIAFID ORCID Logo  ; Gomes, Margarete Z 4 

 Health and Environment Postgraduate Program, Tiradentes University, Aracaju 49032 490, Sergipe, Brazil; [email protected] 
 Biomedicine Graduation Program, Tiradentes University, Aracaju 49032 490, Sergipe, Brazil; [email protected] 
 School of Pharmacy, Tiradentes University, Aracaju 49032 490, Sergipe, Brazil; [email protected] 
 Laboratory of Morphology and Experimental Pathology (LMBE), Institute of Technology and Research (ITP), Tiradentes University, Aracaju 49032 490, Sergipe, Brazil; [email protected] (R.N.P.F.); [email protected] (R.L.C.A.J.); [email protected] (M.Z.G.) 
 Department of Odontology, Paraiba State University, Campina Grande 58429 500, Paraíba, Brazil; [email protected] 
 Department of Pharmaceutical Sciences, Federal University of Sergipe, São Cristóvão 49000 100, Sergipe, Brazil; [email protected] (B.S.L.); [email protected] (A.A.S.A.) 
 Nanomedicine and Nanotechnology Laboratory (LNMed), Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010 390, Sergipe, Brazil; [email protected] 
 Laboratory of Biomaterials (LBmat), Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010 390, Sergipe, Brazil 
 CEB-Centre of Biological Engineering, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal 
First page
4274
Publication year
2021
Publication date
2021
Publisher
MDPI AG
e-ISSN
20734360
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2612844799
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.