Abstract

Rice blast, one of the most destructive epidemic diseases, annually causes severe losses in grain yield worldwide. To manage blast disease, breeding resistant varieties is considered a more economic and environment-friendly strategy than chemical control. For breeding new resistant varieties, natural germplasms with broad-spectrum resistance are valuable resistant donors, but the number is limited. Therefore, artificially induced mutants are an important resource for identifying new broad-spectrum resistant (R) genes/loci. To pursue this approach, we focused on a broad-spectrum blast resistant rice mutant line SA0169, which was previously selected from a sodium azide induced mutation pool of TNG67, an elite japonica variety. We found that SA0169 was completely resistant against the 187 recently collected blast isolates and displayed durable resistance for almost 20 years. Linkage mapping and QTL-seq analysis indicated that a 1.16-Mb region on chromosome 6 (Pi169-6(t)) and a 2.37-Mb region on chromosome 11 (Pi169-11(t)) conferred the blast resistance in SA0169. Sequence analysis and genomic editing study revealed 2 and 7 candidate R genes in Pi169-6(t) and Pi169-11(t), respectively. With the assistance of mapping results, six blast and bacterial blight double resistant lines, which carried Pi169-6(t) and/or Pi169-11(t), were established. The complementation of Pi169-6(t) and Pi169-11(t), like SA0169, showed complete resistance to all tested isolates, suggesting that the combined effects of these two genomic regions largely confer the broad-spectrum resistance of SA0169. The sodium azide induced mutant SA0169 showed broad-spectrum and durable blast resistance. The broad resistance spectrum of SA0169 is contributed by the combined effects of two R regions, Pi169-6(t) and Pi169-11(t). Our study increases the understanding of the genetic basis of the broad-spectrum blast resistance induced by sodium azide mutagenesis, and lays a foundation for breeding new rice varieties with durable resistance against the blast pathogen.

Details

Title
Two genomic regions of a sodium azide induced rice mutant confer broad-spectrum and durable resistance to blast disease
Author
Kuan-Lin, Lo 1 ; Yi-Nian, Chen 2 ; Min-Yu, Chiang 1 ; Mei-Chun, Chen 2 ; Panibe, Jerome P 3 ; Chung-Chun, Chiu 1 ; Lu-Wei, Liu 1 ; Chen Liang-Jwu 4 ; Chun-Wei, Chen 2 ; Li, Wen-Hsiung 5 ; Chang-Sheng, Wang 6   VIAFID ORCID Logo 

 National Chung Hsing University, Department of Agronomy, Taichung, Taiwan (GRID:grid.260542.7) (ISNI:0000 0004 0532 3749) 
 Taiwan Agriculture Research Institute, Division of Plant Pathology, Taichung, Taiwan (GRID:grid.260542.7) 
 National Tsing Hua University, Institute of Molecular and Cellular Biology, Hsinchu, Taiwan (GRID:grid.38348.34) (ISNI:0000 0004 0532 0580); Taiwan International Graduate Program, Institute of Information Science, Academia Sinica, Bioinformatics Program, Taipei, Taiwan (GRID:grid.28665.3f) (ISNI:0000 0001 2287 1366); Academia Sinica, Biodiversity Research Center, Taipei, Taiwan (GRID:grid.28665.3f) (ISNI:0000 0001 2287 1366) 
 National Chung Hsing University, Institute of Molecular Biology, Taichung, Taiwan (GRID:grid.260542.7) (ISNI:0000 0004 0532 3749); National Chung Hsing University, Advanced Plant Biotechnology Center, Taichung, Taiwan (GRID:grid.260542.7) (ISNI:0000 0004 0532 3749) 
 Academia Sinica, Biodiversity Research Center, Taipei, Taiwan (GRID:grid.28665.3f) (ISNI:0000 0001 2287 1366); University of Chicago, Department of Ecology and Evolution, Chicago, USA (GRID:grid.170205.1) (ISNI:0000 0004 1936 7822) 
 National Chung Hsing University, Department of Agronomy, Taichung, Taiwan (GRID:grid.260542.7) (ISNI:0000 0004 0532 3749); National Chung Hsing University, Advanced Plant Biotechnology Center, Taichung, Taiwan (GRID:grid.260542.7) (ISNI:0000 0004 0532 3749) 
Publication year
2022
Publication date
Dec 2022
Publisher
Springer Nature B.V.
ISSN
19398425
e-ISSN
19348037
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2618382721
Copyright
© The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.